Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKV75_RS05190 Genome accession   NZ_LT906456
Coordinates   1134895..1135332 (-) Length   145 a.a.
NCBI ID   WP_014991735.1    Uniprot ID   -
Organism   Actinobacillus suis strain NCTC12996     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1129895..1140332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV75_RS05165 (SAMEA4504061_01065) yacG 1130799..1130987 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  CKV75_RS05170 (SAMEA4504061_01066) coaE 1130969..1131607 (-) 639 WP_014991731.1 dephospho-CoA kinase -
  CKV75_RS05175 (SAMEA4504061_01067) - 1131629..1132303 (-) 675 WP_014991732.1 fimbrial protein -
  CKV75_RS05180 (SAMEA4504061_01068) - 1132303..1133496 (-) 1194 WP_014991733.1 type II secretion system F family protein -
  CKV75_RS05185 (SAMEA4504061_01069) pilB 1133489..1134874 (-) 1386 WP_014991734.1 GspE/PulE family protein Machinery gene
  CKV75_RS05190 (SAMEA4504061_01070) pilA 1134895..1135332 (-) 438 WP_014991735.1 pilin Machinery gene
  CKV75_RS05195 (SAMEA4504061_01071) radA 1135512..1136891 (+) 1380 WP_014991736.1 DNA repair protein RadA Machinery gene
  CKV75_RS05200 (SAMEA4504061_01072) pepB 1136957..1138240 (+) 1284 WP_014991737.1 aminopeptidase PepB -
  CKV75_RS05205 (SAMEA4504061_01073) - 1138368..1139303 (+) 936 WP_014991738.1 KpsF/GutQ family sugar-phosphate isomerase -
  CKV75_RS05210 (SAMEA4504061_01074) greB 1139313..1139801 (+) 489 WP_014991739.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15395.62 Da        Isoelectric Point: 7.6225

>NTDB_id=1147539 CKV75_RS05190 WP_014991735.1 1134895..1135332(-) (pilA) [Actinobacillus suis strain NCTC12996]
MKHIIKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGATNSCFSGANGIKN
GDDLKNSKYLDSVSVTAGTITVNGKGSVAGYGYTLTPDYSSKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1147539 CKV75_RS05190 WP_014991735.1 1134895..1135332(-) (pilA) [Actinobacillus suis strain NCTC12996]
ATGAAACACATAATTAAACCGATACAACAGGCATTTACCCTGATTGAATTAATGATTGTGATTGCAATCATTGCAATTTT
AGCAACTGTGGCGATTCCTTCTTATAACAATTACACCAAAAAAGCGGCATTATCCGAACTGTTAGCGGCATCTTCTGCAT
ATAAAACGGATGTAGAAATTTGTATTTATAACACTGGAGCTACCAATAGCTGTTTTAGTGGAGCCAATGGCATTAAGAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGATTCAGTCTCTGTAACTGCCGGTACTATAACCGTGAATGGCAAGGGCAG
CGTAGCGGGATATGGTTATACACTTACACCGGATTATTCAAGTAAAACACTGACGTGGAAAACAACCTGTTCAGGAGAGG
ATAAAAGTTTATTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)

Pilin (bacterial filament) (Pilin)

(41-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

60.274

100

0.607
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  comP Acinetobacter baylyi ADP1

42.254

97.931

0.414
  pilA/pilA1 Eikenella corrodens VA1

36.552

100

0.366

Multiple sequence alignment