Detailed information NTG1146530

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CFBP1590_RS14445
• Genome accession: NZ_LT855380
• Coordinates: 3200414..3201580 (-)
• Length: 388 a.a.
• NCBI ID: WP_088235328.1
• Uniprot ID: -
• Organism: Pseudomonas viridiflava strain CFBP 1590 isolate E12-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3195414..3206580

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFBP1590_RS14430 (CFBP1590__2922)	-	3196577..3197989 (+)	1413	WP_088235326.1	sigma-54-dependent Fis family transcriptional regulator	-
CFBP1590_RS14435 (CFBP1590__2923)	-	3198241..3199632 (+)	1392	WP_058432729.1	GntP family permease	-
CFBP1590_RS14440 (CFBP1590__2924)	-	3199643..3200401 (+)	759	WP_088235327.1	3-hydroxybutyrate dehydrogenase	-
CFBP1590_RS14445 (CFBP1590__2925)	pilU	3200414..3201580 (-)	1167	WP_088235328.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CFBP1590_RS14450 (CFBP1590__2926)	-	3201692..3201967 (+)	276	WP_004879573.1	peptidylprolyl isomerase	-
CFBP1590_RS14455 (CFBP1590__2927)	pgm	3202024..3203670 (-)	1647	WP_088235329.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
CFBP1590_RS14460 (CFBP1590__2928)	-	3203778..3206048 (-)	2271	WP_088235330.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42775.10 Da
• Isoelectric Point: 6.6698

>NTDB_id=1146530 CFBP1590_RS14445 WP_088235328.1 3200414..3201580(-) (pilU) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGTETLKPGEVAKIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLYLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNQLNELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKHADSQNNVRLRLKLHGENGVNTLTTAPAAPTGSAKPSMVEWGLVDDEPPSQS

Nucleotide

• Length: 1167 bp

>NTDB_id=1146530 CFBP1590_RS14445 WP_088235328.1 3200414..3201580(-) (pilU) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCCACCGGAGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAACCGCTGGGCACCGAAACCCTCAAGCCGGGCGAAGTGGCGAAGATCGCCCAGGGCC
TGATGGATGAAGAGCAGAAGCTGGAGTTCCTGCGCGAGCTGGAAATGAATCTCGCCGTTTCACTGGCGGGCATCGGGCGT
TTCCGGATCAACATCTTCATGCAGCGCAACGAAGTGTCGATCGTGGCGCGTAACATCAAGCTGGACATTCCGCGCTTCGA
AGACCTGTATCTGCCGCCGGTCCTGCTCGACGTGATCATGGAAAAGCACGGCCTGGTGCTGTTCGTCGGTGCCACCGGTT
CGGGTAAGTCCACCTCATTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATTCACCGGCACAAGAAGTCCATCGTCAATCAGCGGGAAGTGGGCGTTGATACCCGCAGTTTCAGGGC
CGCGCTGAAAAATACCCTGCGTCAGGCGCCGGATGTGATTCTGATCGGCGAGATCCGCGACCGTGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGGCACCTGGCGATCTCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGCCGTCCGCAGCTGTTGCATGACCTGGGTAATAACCTCAAGGCGTTCGTGTCCCAGCG
TCTGGTCAAAACCCCGGATGGCAAGCGCCGTGCTGCGGTTGAGGTGATGATGGGCACGCCGACCATTCGTGATCTGATCC
AGCGTAACCAGTTGAACGAGCTCAAAGGGATCATGGAGAAGTCCGGCAGCCTGGGCATGCAGACCTTCGATACTGCGCTG
TTCAACCTTGCGGTGGAGGGCGCGATCAGCGAAGAGGAAGCGCTGAAGCATGCGGATTCGCAGAACAACGTGCGCCTGCG
CCTCAAGTTGCACGGCGAAAACGGAGTGAACACCCTCACGACAGCGCCTGCTGCGCCGACCGGTTCGGCCAAGCCGAGCA
TGGTCGAGTGGGGGCTGGTCGACGACGAGCCCCCGTCCCAGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.065	94.845	0.541
pilU	Acinetobacter baylyi ADP1	51.831	91.495	0.474
pilU	Vibrio cholerae strain A1552	51.143	90.206	0.461
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.51	94.588	0.374
pilT	Pseudomonas aeruginosa PAK	41.108	88.402	0.363
pilT	Pseudomonas stutzeri DSM 10701	40.816	88.402	0.361