Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DABAL43B_RS00385 Genome accession   NZ_LT799838
Coordinates   90104..91813 (-) Length   569 a.a.
NCBI ID   WP_079690563.1    Uniprot ID   A0A1U6GIH4
Organism   Psychrobacter sp. DAB_AL43B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 85104..96813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DABAL43B_RS00360 (DABAL43B_0072) - 85592..85927 (+) 336 WP_079690558.1 pyrimidine/purine nucleoside phosphorylase -
  DABAL43B_RS00365 (DABAL43B_0073) rlmB 86146..86916 (+) 771 WP_079690559.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DABAL43B_RS00370 (DABAL43B_0074) coaE 86879..87571 (-) 693 WP_079690560.1 dephospho-CoA kinase -
  DABAL43B_RS00375 (DABAL43B_0075) pilD 87725..88624 (-) 900 WP_079690561.1 prepilin peptidase Machinery gene
  DABAL43B_RS00380 (DABAL43B_0076) pilC 88650..89873 (-) 1224 WP_079690562.1 type II secretion system F family protein Machinery gene
  DABAL43B_RS00385 (DABAL43B_0077) pilB 90104..91813 (-) 1710 WP_079690563.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DABAL43B_RS00390 (DABAL43B_0078) tpiA 92263..93072 (+) 810 WP_079690564.1 triose-phosphate isomerase -
  DABAL43B_RS00395 (DABAL43B_0079) secG 93289..93597 (+) 309 WP_079692976.1 preprotein translocase subunit SecG -
  DABAL43B_RS00410 (DABAL43B_0082) rimP 94459..94959 (+) 501 WP_079690565.1 ribosome maturation factor RimP -
  DABAL43B_RS00415 (DABAL43B_0083) nusA 95138..96622 (+) 1485 WP_079690566.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63030.38 Da        Isoelectric Point: 5.1558

>NTDB_id=1146231 DABAL43B_RS00385 WP_079690563.1 90104..91813(-) (pilB) [Psychrobacter sp. DAB_AL43B]
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTESQQQQIGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDALSSDVIPK
DLVDEKIIRKFNALPIFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESLYADTMQNFDSFSDSDL
NIGFDEGEEEEGETKLSDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAAR
LKVMSQMDISERRVPQDGRIKLKISKDKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQKEMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGDTNISTAEDPVEINLEGINQVNVNPKVGLTFSSALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGFN
DADLDNPDHTIYEPVGCNECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAAVKNGFRDLRRSGILKVLQGVTSI
QEMMRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1146231 DABAL43B_RS00385 WP_079690563.1 90104..91813(-) (pilB) [Psychrobacter sp. DAB_AL43B]
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCGCAGCAGTTGATCAGCGAAGGTATCGTGAGCGAAGCACATATGAA
AGCGGCGCAGACTGAGTCGCAGCAGCAGCAAATAGGGCTGGTGTCTTATCTAGTAGAGCATAAACTTGCCAACGCTTATC
AATTGGCACAGATGCTGTCGCAAGCTTTTGGCGATCCTTTATTTGATCTTGATGCGCTAAGCTCAGATGTCATCCCAAAA
GATTTGGTTGATGAGAAAATTATCCGCAAATTTAATGCTTTGCCAATCTTTAAGCGTGGACAGCGCTTGTTTGTGGCATT
AAGTGATCCAACACGTATCGACGCTATCGACGCTATTGCCTTTAACTCACGCTTATCTATTGAAACCGTTGTGGTCGAAG
AAGATAAGTTGAAAAGGCGTATTGAGAGCCTTTACGCTGATACGATGCAGAATTTCGATAGCTTTTCTGATAGTGATTTA
AATATTGGCTTTGATGAAGGCGAAGAAGAGGAAGGCGAAACCAAACTCTCTGATAGTGTCGAGGAAGCACCGGTAGTAAA
ATTTGTCAATAAAATGCTGGTTGATGCCATTCGTATGGGAGCCTCTGACTTACATTTCGAGCCCTATGAAAAGAGTTATC
GGGTCCGTTTTCGTGTCGACGGTGTGATGCAAAAAATGGCCAACCCACCCGTACAGCTTGCTGGTAAAATTGCTGCACGC
CTAAAAGTGATGTCACAAATGGACATCTCAGAGCGCCGTGTACCGCAAGATGGACGTATCAAACTTAAAATATCTAAAGA
TAAAGCCATCGACTTTCGGGTAAGCTGTCTACCTACCCTATTTGGTGAAAAACTTGTCCTACGTATCTTAGATCCCTCAT
CAGCCATGTTGGGTATTGAAGCGCTCGGTTATGAGCCCGATCAAAAAGAGATGTTTTTAGAGGCGTTGCATAAACCGCAA
GGAATGCTCCTCATTACTGGGCCAACAGGTTCTGGTAAAACGGTTTCTTTATATACAGGGATTAACATACTTAATACCGG
TGATACTAATATCTCAACCGCTGAAGATCCCGTCGAAATTAACCTTGAAGGGATTAACCAAGTCAACGTTAACCCGAAAG
TGGGACTGACTTTTTCTAGCGCCCTTAAGTCATTCTTACGCCAAGATCCCGATATCGTAATGGTTGGTGAGATTCGTGAC
CTTGAAACGGCCGAGATTGCTATCAAAGCCGCGCAAACAGGCCATATGGTACTCTCAACCCTTCATACTAACTCTGCGCC
AGAGACCTTAACGCGTCTACGCAATATGGGCGTGGCTTCTTTTAATATCGCGACTTCCGTGAATTTGGTCATTGCCCAGC
GGCTAGCACGACGCTTATGTAAGAACTGTAAAAAGCCTATCAATATCCCACGTCAAAGCTTGCTTGAGCTTGGCTTTAAC
GATGCTGACTTGGACAATCCAGACCATACTATTTATGAACCCGTAGGCTGTAATGAATGCCGCGAAGGCTACAAGGGCCG
TGTCGGTATTTATGAAGTCATGAAAGTCAGCCCTGATATTTCACGTATCATCATGGAAGACGGTAACGCGATAGATATCA
AAGATGCCGCAGTTAAAAATGGTTTTAGAGACTTACGCCGTTCTGGTATTTTAAAAGTTTTACAGGGCGTCACCAGTATT
CAAGAAATGATGCGCGTCACCTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U6GIH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.07

100

0.682
  pilB Acinetobacter baylyi ADP1

64.374

99.649

0.641
  pilB Legionella pneumophila strain ERS1305867

51.736

100

0.524
  pilF Neisseria gonorrhoeae MS11

50.442

99.297

0.501
  pilB Vibrio cholerae strain A1552

49.195

98.243

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.818

100

0.482
  pilB Vibrio campbellii strain DS40M4

48.022

97.715

0.469

Multiple sequence alignment