Detailed information NTG1146229

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DABAL43B_RS00375
• Genome accession: NZ_LT799838
• Coordinates: 87725..88624 (-)
• Length: 299 a.a.
• NCBI ID: WP_079690561.1
• Uniprot ID: A0A1U6GIE3
• Organism: Psychrobacter sp. DAB_AL43B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 82725..93624

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DABAL43B_RS00355 (DABAL43B_0071)	recN	83343..85022 (-)	1680	WP_079690556.1	DNA repair protein RecN	-
DABAL43B_RS00360 (DABAL43B_0072)	-	85592..85927 (+)	336	WP_079690558.1	pyrimidine/purine nucleoside phosphorylase	-
DABAL43B_RS00365 (DABAL43B_0073)	rlmB	86146..86916 (+)	771	WP_079690559.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
DABAL43B_RS00370 (DABAL43B_0074)	coaE	86879..87571 (-)	693	WP_079690560.1	dephospho-CoA kinase	-
DABAL43B_RS00375 (DABAL43B_0075)	pilD	87725..88624 (-)	900	WP_079690561.1	prepilin peptidase	Machinery gene
DABAL43B_RS00380 (DABAL43B_0076)	pilC	88650..89873 (-)	1224	WP_079690562.1	type II secretion system F family protein	Machinery gene
DABAL43B_RS00385 (DABAL43B_0077)	pilB	90104..91813 (-)	1710	WP_079690563.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DABAL43B_RS00390 (DABAL43B_0078)	tpiA	92263..93072 (+)	810	WP_079690564.1	triose-phosphate isomerase	-
DABAL43B_RS00395 (DABAL43B_0079)	secG	93289..93597 (+)	309	WP_079692976.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 32848.40 Da
• Isoelectric Point: 8.4777

>NTDB_id=1146229 DABAL43B_RS00375 WP_079690561.1 87725..88624(-) (pilD) [Psychrobacter sp. DAB_AL43B]
MQFIQLLQENMALALIIFGLLGLCVGSFLNVVIHRIPLMMISAWRQECSQFMSQQADMPHEHTVPLADIVATDTPITLSR
PASRCPHCAHKIKWYENIPLISWMVLRGRCSDCKAAIGLRYPIVELVTALLSVLVIYKFGVSAAGTSALVLVWTLVALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVSPTQSIWGLLLGFLSLWIVVKVFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGIILMKKQGESRPFAFGPYIAIAGIVALLYGSDIVSWYLGMYT

Nucleotide

• Length: 900 bp

>NTDB_id=1146229 DABAL43B_RS00375 WP_079690561.1 87725..88624(-) (pilD) [Psychrobacter sp. DAB_AL43B]
ATGCAGTTTATACAATTATTACAAGAAAATATGGCTTTAGCTTTAATCATATTTGGTCTGCTAGGTCTTTGTGTGGGCAG
TTTTTTAAATGTGGTCATTCACCGTATTCCGCTCATGATGATCTCTGCTTGGCGACAAGAATGTAGCCAGTTTATGTCTC
AACAAGCAGATATGCCGCATGAACATACTGTGCCTCTAGCCGATATCGTCGCCACTGATACACCTATTACTTTAAGTAGA
CCGGCATCACGCTGTCCTCACTGCGCACATAAAATTAAGTGGTACGAAAATATACCTTTAATTAGCTGGATGGTATTACG
CGGACGCTGCTCAGATTGTAAAGCCGCTATCGGACTGCGTTATCCCATTGTTGAGCTGGTTACTGCACTTCTCTCTGTAT
TGGTCATTTATAAATTTGGCGTCAGTGCAGCTGGAACATCAGCATTAGTTTTAGTATGGACACTCGTTGCGTTAACCGGT
ATTGATTTTGATACGCAGCTACTACCTGACCGCTTAACGTTCCCATTAGCAGGGTTAGGATTGGCCGTAAACTCGCAGGG
TTGGTTTGTATCGCCAACTCAGTCAATTTGGGGCTTATTGCTTGGGTTTCTATCATTGTGGATAGTGGTTAAGGTGTTCT
ATTTAATCACTAAAAAACATGGTATGGGACAAGGAGACTTTAAATTATTAGCAGTATTGGGTGCTTGGCTTGGACCCATG
ATGTTGCCGCTAATTATTCTATTATCATCCTTGCTCGGCTCAATCGTTGGAATCATTCTCATGAAGAAACAAGGCGAAAG
CAGACCTTTTGCCTTTGGCCCTTATATCGCTATTGCCGGTATTGTGGCTTTACTGTACGGCTCAGATATCGTCAGCTGGT
ATTTAGGTATGTATACTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-139)

• Type IV leader peptidase family (Peptidase_A24) (149-258)

3D structure

• AlphaFold DB: A0A1U6GIE3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	55.017	96.656	0.532
pilD	Acinetobacter baumannii D1279779	54.325	96.656	0.525
pilD	Vibrio cholerae strain A1552	47.312	93.311	0.441
pilD	Vibrio campbellii strain DS40M4	44.444	99.331	0.441
pilD	Neisseria gonorrhoeae MS11	40.559	95.652	0.388