Detailed information NTG1145952

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BQ6873_RS09695
• Genome accession: NZ_LT671418
• Coordinates: 2059614..2060750 (+)
• Length: 378 a.a.
• NCBI ID: WP_076592466.1
• Uniprot ID: -
• Organism: Herminiimonas arsenitoxidans strain AS8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2054614..2065750

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BQ6873_RS09665	-	2055055..2055696 (+)	642	WP_076592460.1	DJ-1/PfpI family protein	-
BQ6873_RS09670	-	2055712..2056212 (+)	501	WP_076592461.1	GNAT family N-acetyltransferase	-
BQ6873_RS09675	-	2056424..2056837 (+)	414	WP_076592462.1	RcnB family protein	-
BQ6873_RS09680	proC	2056919..2057743 (-)	825	WP_076592463.1	pyrroline-5-carboxylate reductase	-
BQ6873_RS09685	-	2057733..2058437 (-)	705	WP_076592464.1	YggS family pyridoxal phosphate-dependent enzyme	-
BQ6873_RS09690	pilT	2058534..2059577 (+)	1044	WP_076592465.1	type IV pilus twitching motility protein PilT	Machinery gene
BQ6873_RS09695	pilU	2059614..2060750 (+)	1137	WP_076592466.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BQ6873_RS09700	-	2060747..2061232 (+)	486	WP_076592467.1	glutathione peroxidase	-
BQ6873_RS09705	glcF	2061308..2062540 (-)	1233	WP_076592468.1	glycolate oxidase subunit GlcF	-
BQ6873_RS09710	glcE	2062641..2063696 (-)	1056	WP_076592469.1	glycolate oxidase subunit GlcE	-
BQ6873_RS09715	-	2063712..2065199 (-)	1488	WP_076594054.1	FAD-linked oxidase C-terminal domain-containing protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42019.46 Da
• Isoelectric Point: 6.6619

>NTDB_id=1145952 BQ6873_RS09695 WP_076592466.1 2059614..2060750(+) (pilU) [Herminiimonas arsenitoxidans strain AS8]
MERDQATKFMYDLLRLMLSKNGSDLFVTADFPPAFKIDGKMTQVSNQPLTAAHTIELARAIMSDKQAAEFEATKECNFAI
SPGGIGRFRVSAFVQQGRVGLVIRTITTAIPKIEDLGVPETLKDIVMTKRGLVIMVGATGSGKSTTLAAMIGHRNEHSYG
HIITIEDPVEYVHPHKNCVITQREIGVDTDGWGIALKNTLRQAPDVIFIGEIRDRETMDYAIAFAETGHLCLATMHANSS
NQALDRIVNFFPEERRQQLLMDLSLNLKAMISQRLIPLKDTKGRCVAVEIMLNSPLVSDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKISYEDALRNADSVNDLRLSIKLNGKEAQTRDLSKGTEHLGIV

Nucleotide

• Length: 1137 bp

>NTDB_id=1145952 BQ6873_RS09695 WP_076592466.1 2059614..2060750(+) (pilU) [Herminiimonas arsenitoxidans strain AS8]
ATGGAACGCGATCAAGCCACTAAATTCATGTACGACTTGCTCCGCCTAATGCTGAGCAAGAACGGCTCCGATCTTTTCGT
CACAGCTGACTTCCCGCCTGCATTCAAGATCGACGGCAAGATGACGCAAGTATCAAATCAACCGTTGACAGCTGCACACA
CGATTGAATTGGCGCGCGCCATCATGAGCGACAAGCAAGCGGCAGAATTTGAAGCGACGAAAGAATGTAATTTCGCGATC
AGCCCAGGTGGCATCGGTCGCTTCCGCGTGTCGGCATTCGTGCAGCAAGGTCGCGTCGGCTTGGTCATCCGCACCATTAC
GACGGCCATACCAAAAATTGAAGATCTAGGCGTACCGGAAACCCTGAAAGATATCGTGATGACCAAGCGCGGTCTGGTCA
TCATGGTGGGTGCAACTGGCTCAGGCAAATCGACTACTTTGGCCGCCATGATCGGCCACCGCAACGAACACAGCTACGGC
CACATCATCACGATCGAAGATCCGGTTGAATACGTGCATCCGCATAAAAACTGCGTCATCACGCAACGCGAAATCGGTGT
CGATACCGATGGTTGGGGTATCGCACTGAAGAACACTTTACGTCAGGCACCTGATGTTATTTTCATTGGCGAGATACGTG
ATCGCGAAACCATGGATTACGCGATCGCTTTTGCCGAAACCGGTCATCTGTGTTTGGCGACCATGCATGCGAATAGCTCG
AATCAAGCGCTGGATCGTATCGTCAACTTCTTCCCGGAAGAACGTCGCCAGCAATTGCTGATGGATTTGTCGTTGAATTT
GAAAGCGATGATTTCGCAACGCCTGATTCCGCTGAAAGATACCAAAGGCCGCTGTGTCGCAGTAGAGATCATGTTGAACT
CGCCGCTCGTCTCCGACCTGATCTTCAAAGGCGATGTGCACGAGATCAAAGAGATCATGAAGAAGTCACGCGAACTCGGC
ATGCAGACTTTCGATCAAGCTTTATTTGATTTGTATGAAGCAGGAAAAATTTCTTACGAAGATGCACTCCGCAACGCCGA
CTCGGTCAACGATTTGCGCCTTTCCATCAAATTGAATGGCAAGGAAGCACAGACGCGCGATCTGAGCAAAGGCACTGAAC
ATTTGGGTATCGTATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.606	96.032	0.582
pilU	Acinetobacter baylyi ADP1	59.599	92.328	0.55
pilU	Vibrio cholerae strain A1552	55.132	90.212	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.478	88.624	0.394
pilT	Pseudomonas aeruginosa PAK	41.493	88.624	0.368