Detailed information NTG1144876

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLA12_RS09285
• Genome accession: NZ_LT599049
• Coordinates: 1943302..1943949 (-)
• Length: 215 a.a.
• NCBI ID: WP_021722185.1
• Uniprot ID: S6FFJ3
• Organism: Lactococcus lactis subsp. lactis strain A12
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1938302..1948949

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLA12_RS09260 (LLA12_01974)	atpF	1938372..1938878 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLA12_RS09265 (LLA12_01975)	atpB	1938893..1939606 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLA12_RS09270 (LLA12_01976)	-	1939651..1939866 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLA12_RS09275 (LLA12_01977)	-	1940051..1940827 (-)	777	WP_021722183.1	alpha/beta hydrolase	-
LLA12_RS09280 (LLA12_01979)	-	1941112..1943321 (-)	2210	Protein_1827	DNA internalization-related competence protein ComEC/Rec2	-
LLA12_RS09285 (LLA12_01980)	comEA	1943302..1943949 (-)	648	WP_021722185.1	ComEA family DNA-binding protein	Machinery gene
LLA12_RS09290 (LLA12_01981)	-	1944009..1945370 (-)	1362	WP_021722186.1	ABC transporter permease	-
LLA12_RS09295 (LLA12_01982)	-	1945367..1946299 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLA12_RS09300 (LLA12_01983)	-	1946404..1946748 (-)	345	WP_230473238.1	hypothetical protein	-
LLA12_RS09305 (LLA12_01984)	-	1946906..1947469 (-)	564	WP_021722188.1	GNAT family N-acetyltransferase	-
LLA12_RS09310 (LLA12_01985)	-	1947642..1948820 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23637.80 Da
• Isoelectric Point: 4.9944

>NTDB_id=1144876 LLA12_RS09285 WP_021722185.1 1943302..1943949(-) (comEA) [Lactococcus lactis subsp. lactis strain A12]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTNQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1144876 LLA12_RS09285 WP_021722185.1 1943302..1943949(-) (comEA) [Lactococcus lactis subsp. lactis strain A12]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTAACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-212)

• SLBB domain (SLBB) (70-125)

3D structure

• AlphaFold DB: S6FFJ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	35.683	100	0.377