Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A8Q77_RS07255 Genome accession   NZ_LT571436
Coordinates   1573743..1575422 (-) Length   559 a.a.
NCBI ID   WP_004282574.1    Uniprot ID   -
Organism   Neisseria weaveri strain NCTC13585     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568743..1580422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8Q77_RS10375 (SAMEA3174300_01450) yacG 1570032..1570214 (-) 183 WP_081463174.1 DNA gyrase inhibitor YacG -
  A8Q77_RS07235 (SAMEA3174300_01451) zapD 1570220..1570993 (-) 774 WP_004282578.1 cell division protein ZapD -
  A8Q77_RS07240 (SAMEA3174300_01452) coaE 1570990..1571613 (-) 624 WP_064130789.1 dephospho-CoA kinase -
  A8Q77_RS07245 (SAMEA3174300_01453) pilD 1571615..1572475 (-) 861 WP_004285616.1 prepilin peptidase Machinery gene
  A8Q77_RS07250 (SAMEA3174300_01454) pilG 1572478..1573719 (-) 1242 WP_004282575.1 type II secretion system F family protein Machinery gene
  A8Q77_RS07255 (SAMEA3174300_01455) pilF 1573743..1575422 (-) 1680 WP_004282574.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8Q77_RS07260 (SAMEA3174300_01456) comE 1575649..1575954 (-) 306 WP_064130790.1 ComEA family DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62286.84 Da        Isoelectric Point: 6.7673

>NTDB_id=1143806 A8Q77_RS07255 WP_004282574.1 1573743..1575422(-) (pilF) [Neisseria weaveri strain NCTC13585]
MSVGLLRVLVQSQILDESKVAKYQNAAQNHKNVISMLFEDGITTPKTLSELLARVFSYPLLDLRHYPKINIVNDILTEEQ
MLQNRCVPIVKKGRKVYLAVSDPTQIQNFQKIAFSADVSIDLLIVRDDQLSALLEWISQRSTTILKEISKEQESVSPSQT
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNTDAGTLNINQLGLETFQKEILLEAIQRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNINEKQGLTFAAALKAFLRQDPDVIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSVIMAQRLLRRLCPHCKAEMERPPVPALKKVGFTDNDLASDWKL
YRSVGCDSCRGKGYKGRVGVYEVMPISENMQRVIMENGSEVDITNIAYQEGMVDLRRSGLLKVMQGITSLEEVLANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1143806 A8Q77_RS07255 WP_004282574.1 1573743..1575422(-) (pilF) [Neisseria weaveri strain NCTC13585]
ATGAGCGTTGGTTTGTTGAGAGTTTTGGTTCAGAGCCAGATCTTGGATGAAAGTAAAGTTGCCAAGTATCAGAATGCTGC
TCAGAATCATAAGAATGTTATTTCCATGTTGTTTGAAGATGGGATTACAACGCCGAAAACACTGAGCGAGTTATTAGCTA
GAGTATTTAGTTATCCTTTGTTGGACTTACGTCATTATCCGAAGATTAATATTGTTAATGACATCTTAACAGAAGAACAA
ATGCTCCAAAATAGATGTGTACCTATTGTTAAAAAGGGTAGAAAAGTCTATTTAGCTGTTTCTGATCCCACTCAAATTCA
GAATTTTCAAAAAATTGCTTTTTCAGCGGATGTTTCGATTGATTTGCTGATTGTACGTGATGATCAGTTAAGCGCATTAT
TGGAATGGATTAGTCAGCGTTCTACAACAATTTTAAAGGAAATCAGTAAAGAGCAAGAATCTGTTTCACCGTCTCAAACA
TTATATATAGATAATGAAGAGGCTGAAGATGGGCCTATAGCAAGGTTTATTCATAAAACGCTTTCAGATGCTTTAAATGC
AGGTGCATCAGATATTCATTTTGAATTTTATGAACAAATGGCCAGGGTACGTTTTAGGGTTGATGGGCAATTGCGTGAAG
TGGTTCAGCCTCCTGTTGCAGTGCGAGGACAATTGGCTTCACGTATTAAAGTGATGGCACGCTTGGATATTTCTGAAAAA
AGAATTCCCCAAGATGGTCGTATTCAAATCGCTTTTCACAAGCATGGGAGACCTATTGACTTCCGTGTTAGTACATTACC
CACTTTATTTGGTGAAAAGGTGGTAATGCGTATTTTAAATACAGATGCAGGAACGCTTAATATTAATCAACTGGGTTTGG
AGACATTTCAAAAAGAGATTTTATTAGAAGCTATTCAGCGTCCGTATGGTATGGTGTTGGTTACTGGGCCTACAGGTTCA
GGTAAAACAGTTTCTTTATATACTTGTTTAAATATTTTAAATACAGAAAATGTTAATATTGCAACGGCTGAGGATCCTGC
AGAGATTAATCTGCCAGGAATTAATCAAGTTAACATTAATGAAAAGCAAGGACTTACTTTTGCGGCTGCGTTGAAAGCAT
TTTTACGTCAGGATCCTGATGTGATTATGGTGGGTGAGATACGAGATTTGGAAACTGCAGATATTGCCATTAAAGCAGCT
CAGACTGGCCATATGGTTTTTTCTACCTTGCACACGAATAATGCTCCGGCAACTTTGTCCCGTATGTTAAATATGGGTGT
AGCGCCGTTTAACATCGCTAGTTCTGTTAGCGTGATTATGGCACAGCGTTTGCTTAGAAGGTTATGTCCGCATTGTAAGG
CTGAAATGGAACGACCTCCTGTGCCTGCATTGAAAAAGGTGGGATTCACCGACAATGATTTGGCTTCCGATTGGAAGCTA
TATAGGTCTGTGGGATGTGACTCTTGTAGAGGTAAAGGCTATAAAGGTCGGGTTGGTGTGTATGAAGTGATGCCTATAAG
CGAAAATATGCAACGTGTGATTATGGAAAATGGTAGTGAGGTTGATATAACGAATATTGCCTACCAGGAAGGCATGGTTG
ATTTACGTAGATCAGGATTGCTAAAAGTAATGCAGGGTATTACTTCTTTAGAAGAAGTTTTAGCCAATACTAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-138)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.313

100

0.753
  pilB Acinetobacter baumannii D1279779

49.64

99.463

0.494
  pilB Acinetobacter baylyi ADP1

49.101

99.463

0.488
  pilB Legionella pneumophila strain ERS1305867

46.429

100

0.465
  pilB Vibrio cholerae strain A1552

45.196

100

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

44.841

95.349

0.428
  pilB Vibrio campbellii strain DS40M4

43.833

94.275

0.413

Multiple sequence alignment