Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   STN4L_RS08115 Genome accession   NZ_LS974444
Coordinates   1525987..1527087 (+) Length   366 a.a.
NCBI ID   WP_120764773.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain N4L     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1520987..1532087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STN4L_RS08105 (STN4L_01902) - 1524721..1525083 (+) 363 WP_014608746.1 DUF1033 family protein -
  STN4L_RS08110 (STN4L_01903) comGA/cglA/cilD 1525164..1526105 (+) 942 WP_014608745.1 competence type IV pilus ATPase ComGA Machinery gene
  STN4L_RS08115 (STN4L_01904) comYB 1525987..1527087 (+) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  STN4L_RS08120 (STN4L_01905) comYC 1527084..1527410 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  STN4L_RS08125 (STN4L_01906) comYD 1527370..1527798 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  STN4L_RS08130 (STN4L_01907) comGE 1527770..1528063 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  STN4L_RS08135 (STN4L_01908) comYF 1528047..1528484 (+) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  STN4L_RS08140 (STN4L_01909) comGG 1528462..1528779 (+) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  STN4L_RS08145 (STN4L_01910) comYH 1528824..1529780 (+) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  STN4L_RS08150 (STN4L_01911) - 1529836..1531029 (+) 1194 WP_014608740.1 acetate kinase -
  STN4L_RS08155 - 1531278..1531476 (+) 199 Protein_1510 helix-turn-helix transcriptional regulator -
  STN4L_RS10620 (STN4L_01913) - 1531488..1531763 (+) 276 WP_011681681.1 hypothetical protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41876.03 Da        Isoelectric Point: 10.1377

>NTDB_id=1143049 STN4L_RS08115 WP_120764773.1 1525987..1527087(+) (comYB) [Streptococcus thermophilus strain N4L]
MPEKISKTIRRPTGISSWKVWLNKDVSLRGISKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKETSLSLM
KESLIRGDRLYQMLALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKNNWAVQLVQIFPQLFFVSLCGLLVLGLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLLGQGVDLLDLVALMQEQKSKLFRELGADLEEALMLGQSFPERIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIVAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1143049 STN4L_RS08115 WP_120764773.1 1525987..1527087(+) (comYB) [Streptococcus thermophilus strain N4L]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTCATCCAGCTCTTCAAACAACTTTTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAACATCCTTGTCTCTTATG
AAAGAGAGTTTAATACGCGGTGATAGGTTGTATCAGATGTTAGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGAGGTGGCGACTTATCCCATACTACTTCTGGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAAGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTTTT
TTTTGTGAGTTTGTGTGGACTCCTTGTGTTGGGTTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCAAAATCCCTTTTATTGGTCAGACAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTATTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAAAAGTCTAAACTCTTCCGTGAGCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCTGAACGTATTGCCAGTCATCCGTTTTTTACTAAGG
AGCTCTCACTAATTATTGCTTATGGGGAGGCCAATGCGAGGTTGGGCTATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCAGTTGTGATTGT
AATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.678

93.443

0.511
  comYB Streptococcus mutans UA159

54.678

93.443

0.511
  comYB Streptococcus gordonii str. Challis substr. CH1

51.17

93.443

0.478
  comGB/cglB Streptococcus mitis NCTC 12261

50.453

90.437

0.456
  comGB/cglB Streptococcus mitis SK321

49.697

90.164

0.448
  comGB/cglB Streptococcus pneumoniae Rx1

49.695

89.617

0.445
  comGB/cglB Streptococcus pneumoniae D39

49.695

89.617

0.445
  comGB/cglB Streptococcus pneumoniae R6

49.695

89.617

0.445
  comGB/cglB Streptococcus pneumoniae TIGR4

49.695

89.617

0.445
  comGB Lactococcus lactis subsp. cremoris KW2

45.758

90.164

0.413

Multiple sequence alignment