Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN91_RS18305 Genome accession   NZ_LS483441
Coordinates   3986646..3987755 (+) Length   369 a.a.
NCBI ID   WP_010673049.1    Uniprot ID   A0AAJ6CRB8
Organism   Aeromonas caviae strain NCTC12244     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3981646..3992755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN91_RS18270 (NCTC12244_03659) - 3981680..3982510 (+) 831 WP_042864458.1 CPBP family intramembrane glutamic endopeptidase -
  DQN91_RS18275 (NCTC12244_03660) - 3982587..3983006 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  DQN91_RS18280 (NCTC12244_03661) yggU 3983025..3983324 (-) 300 WP_041210939.1 DUF167 family protein YggU -
  DQN91_RS18285 (NCTC12244_03662) - 3983324..3983875 (-) 552 WP_042014922.1 YggT family protein -
  DQN91_RS18290 (NCTC12244_03663) proC 3983899..3984723 (-) 825 WP_042014923.1 pyrroline-5-carboxylate reductase -
  DQN91_RS18295 (NCTC12244_03664) - 3984847..3985548 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  DQN91_RS18300 (NCTC12244_03665) pilT 3985588..3986622 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  DQN91_RS18305 (NCTC12244_03666) pilU 3986646..3987755 (+) 1110 WP_010673049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN91_RS18310 (NCTC12244_03667) yaaA 3987788..3988561 (+) 774 WP_042014926.1 peroxide stress protein YaaA -
  DQN91_RS18315 (NCTC12244_03668) srmB 3988749..3989969 (-) 1221 WP_042014927.1 ATP-dependent RNA helicase SrmB -
  DQN91_RS18320 (NCTC12244_03669) - 3990097..3990807 (+) 711 WP_042014929.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  DQN91_RS18325 (NCTC12244_03670) brnQ 3990998..3992305 (+) 1308 WP_080767887.1 branched-chain amino acid transport system II carrier protein -
  DQN91_RS18330 (NCTC12244_03671) - 3992395..3992622 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41000.18 Da        Isoelectric Point: 6.4409

>NTDB_id=1141618 DQN91_RS18305 WP_010673049.1 3986646..3987755(+) (pilU) [Aeromonas caviae strain NCTC12244]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLRPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1141618 DQN91_RS18305 WP_010673049.1 3986646..3987755(+) (pilU) [Aeromonas caviae strain NCTC12244]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGCCTGGTGTCGCTCAGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCCCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGACACCTCTGCCTCGCCACCTTGCACGCAAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TTCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCA
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTATCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.56

91.057

0.388
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment