Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DQN79_RS01815 Genome accession   NZ_LS483435
Coordinates   363589..365268 (-) Length   559 a.a.
NCBI ID   WP_041961550.1    Uniprot ID   -
Organism   Neisseria elongata subsp. glycolytica strain NCTC11050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 358589..370268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN79_RS01780 (NCTC11050_00340) - 359156..359374 (+) 219 WP_003769492.1 SlyX family protein -
  DQN79_RS01785 (NCTC11050_00341) rpmE 359509..359727 (+) 219 WP_081456052.1 50S ribosomal protein L31 -
  DQN79_RS01790 - 359880..360062 (-) 183 WP_234404466.1 DNA gyrase inhibitor YacG -
  DQN79_RS01795 (NCTC11050_00342) zapD 360074..360835 (-) 762 WP_003769485.1 cell division protein ZapD -
  DQN79_RS01800 coaE 360850..361452 (-) 603 WP_041961548.1 dephospho-CoA kinase -
  DQN79_RS01805 (NCTC11050_00344) pilD 361452..362333 (-) 882 WP_003769479.1 prepilin peptidase Machinery gene
  DQN79_RS01810 (NCTC11050_00345) pilG 362341..363564 (-) 1224 WP_041961549.1 type II secretion system F family protein Machinery gene
  DQN79_RS01815 (NCTC11050_00346) pilF 363589..365268 (-) 1680 WP_041961550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DQN79_RS01820 (NCTC11050_00347) - 365403..366992 (-) 1590 WP_003769468.1 inorganic phosphate transporter -
  DQN79_RS01825 (NCTC11050_00348) - 367426..368727 (+) 1302 WP_003769467.1 ATP-binding protein -
  DQN79_RS01830 (NCTC11050_00349) - 368942..369931 (+) 990 WP_003769465.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62260.61 Da        Isoelectric Point: 5.7232

>NTDB_id=1141389 DQN79_RS01815 WP_041961550.1 363589..365268(-) (pilF) [Neisseria elongata subsp. glycolytica strain NCTC11050]
MSMGLLRVLIQNKTLSSEQAGRYQQALKDNKDIIAMLFADGITNPKALAELLAGMFNYPVLDLNYYPRSNIVQDILNEEQ
MQANACVPIFKRGNKIFLAVSDPTRIQEFQKVVFASGFQIDLVIVRHDQLMTLVEWLGQRSTTILREMSQDQLDVGASQS
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAIRGQLASRIKVMARLDISEK
RIPQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEDFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAS
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKEPMERPHEHALKKAGFSDEDLAKDWTM
YRAVGCDTCRGKGYKGRAGVYEVMPITDEMQRVIMEGGTEVDIQKMAYQEGMVDLRRAGLLKVMSGITSLEEVLANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1141389 DQN79_RS01815 WP_041961550.1 363589..365268(-) (pilF) [Neisseria elongata subsp. glycolytica strain NCTC11050]
ATGAGTATGGGATTATTAAGGGTTTTGATTCAGAATAAAACCCTCAGCAGTGAACAAGCTGGACGCTATCAACAAGCATT
GAAAGATAATAAAGATATTATTGCAATGTTGTTTGCTGACGGAATAACTAATCCCAAAGCATTGGCAGAATTGTTGGCCG
GGATGTTCAATTACCCTGTTCTTGATTTGAATTACTATCCGCGTAGTAATATTGTGCAGGATATTTTAAATGAGGAGCAA
ATGCAGGCTAATGCGTGTGTTCCTATATTTAAACGTGGGAATAAGATATTCCTTGCAGTGTCAGATCCAACAAGAATCCA
AGAATTCCAGAAAGTGGTATTTGCTTCAGGATTTCAAATAGATTTGGTAATTGTTCGTCATGACCAGTTAATGACGTTGG
TTGAATGGCTTGGACAGAGGTCTACCACTATCTTGCGTGAGATGTCTCAGGATCAGTTGGATGTGGGGGCATCGCAATCA
TTATATATCGATAATGAAGAGGCCGAGGATGGGCCTATTGCGCGGTTTATTCATAAAACCCTATCTGATGCATTAAATGC
AGGTGCTTCTGATATCCATTTTGAGTTTTATGAGCAGATGGCACGTGTGCGGTTCCGTGTGGATGGGCAGTTGCGAGAAG
TTGTGCAACCTCCTGTTGCAATACGTGGGCAATTGGCGTCACGTATTAAGGTGATGGCAAGATTGGATATTTCTGAAAAA
CGTATTCCTCAAGATGGACGCATCCAAATCGCTTTTTCTAAACATGGTAGACCCACAGATTTTCGTGTGAGTACATTGCC
AACGTTGTTTGGTGAAAAAGTTGTGATGCGTATTCTTAATTCGGATGCAGCATCGCTAAATATTGATCAGCTCGGGTTTG
AAGATTTTCAAAAGGAATTATTACTGGAGGCAATCCATCGGCCATATGGGATGGTTTTAGTAACTGGTCCGACAGGCTCT
GGTAAAACTGTTTCTTTGTATACTTGTCTAAACATTTTAAATACCGAAGGAGTAAATATTTCCACAGCAGAAGATCCGGC
GGAAATTAATTTGCCTGGAATTAACCAAGTAAATGTTAATGATAAACAAGGATTAACTTTTGCAGCTGCACTAAAATCGT
TTCTGCGCCAAGATCCGGATATTATTATGGTTGGTGAGATTCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCATCG
CAGACAGGGCATATGGTGTTTTCTACATTGCATACCAATAATGCTCCTGCAACATTGTCTCGTATGCTGAATATGGGTGT
CGCACCATTTAATATAGCCAGTTCCGTTAGTCTCATTATGGCACAAAGGTTATTGCGTAGATTGTGCCCAAATTGTAAGG
AACCAATGGAAAGGCCTCATGAGCATGCCTTAAAAAAGGCAGGCTTTTCAGATGAAGATTTAGCTAAAGATTGGACAATG
TATCGAGCGGTAGGTTGTGATACATGCAGAGGTAAAGGTTATAAGGGGCGTGCTGGTGTATATGAAGTAATGCCAATTAC
AGATGAAATGCAAAGAGTGATTATGGAAGGTGGAACAGAAGTGGATATTCAAAAAATGGCATATCAAGAGGGAATGGTAG
ATTTACGCCGAGCAGGTTTATTGAAGGTAATGAGCGGCATTACTTCGTTAGAGGAAGTATTAGCTAATACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.671

100

0.757
  pilB Acinetobacter baumannii D1279779

51.601

100

0.519
  pilB Acinetobacter baylyi ADP1

50.626

100

0.506
  pilB Legionella pneumophila strain ERS1305867

47.95

100

0.481
  pilB Vibrio cholerae strain A1552

47.963

96.601

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.008

94.275

0.453
  pilB Vibrio campbellii strain DS40M4

46.49

94.275

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

93.739

0.369

Multiple sequence alignment