Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN52_RS07030 Genome accession   NZ_LS483426
Coordinates   1314558..1315742 (-) Length   394 a.a.
NCBI ID   WP_003786168.1    Uniprot ID   -
Organism   Kingella kingae strain NCTC10529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309558..1320742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS06990 (NCTC10529_01361) tatC 1310499..1311263 (-) 765 WP_003786159.1 twin-arginine translocase subunit TatC -
  DQN52_RS06995 (NCTC10529_01362) tatB 1311260..1311766 (-) 507 WP_003786160.1 Sec-independent protein translocase protein TatB -
  DQN52_RS07000 (NCTC10529_01363) tatA 1311768..1311971 (-) 204 WP_003786162.1 Sec-independent protein translocase subunit TatA -
  DQN52_RS07005 (NCTC10529_01364) - 1312023..1312343 (-) 321 WP_003786163.1 histidine triad nucleotide-binding protein -
  DQN52_RS07010 (NCTC10529_01365) - 1312425..1312745 (-) 321 WP_003786164.1 phosphoribosyl-ATP diphosphatase -
  DQN52_RS07015 (NCTC10529_01366) hisI 1312833..1313169 (-) 337 Protein_1333 phosphoribosyl-AMP cyclohydrolase -
  DQN52_RS07020 (NCTC10529_01367) - 1313179..1313709 (-) 531 WP_003786166.1 CreA family protein -
  DQN52_RS07025 (NCTC10529_01368) - 1313720..1314475 (-) 756 WP_003786167.1 sulfite exporter TauE/SafE family protein -
  DQN52_RS07030 (NCTC10529_01369) pilU 1314558..1315742 (-) 1185 WP_003786168.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN52_RS07035 (NCTC10529_01370) - 1315924..1316406 (+) 483 WP_003786169.1 Lrp/AsnC family transcriptional regulator -
  DQN52_RS07040 (NCTC10529_01371) - 1316512..1317852 (+) 1341 WP_003786170.1 DUF3482 domain-containing protein -
  DQN52_RS07045 (NCTC10529_01372) - 1318015..1318860 (+) 846 WP_003786171.1 PHP domain-containing protein -
  DQN52_RS07050 (NCTC10529_01373) - 1318983..1320434 (+) 1452 WP_032827644.1 sodium:proton antiporter -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43758.27 Da        Isoelectric Point: 6.0102

>NTDB_id=1141190 DQN52_RS07030 WP_003786168.1 1314558..1315742(-) (pilU) [Kingella kingae strain NCTC10529]
MQVIIPPINEEMRKFIHGLLTHMVNNKGSDLFITAGYPPAMKLDGKLTKITDKPLTADHAMQIARSIMDDKQAEEFLTTN
ECNFAISLPGVSRFRINAMVQRGAAALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMAT
LHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKKQGKGRVAAVEILLNSPLISELVLHGDIHAVKEIMA
KSRDIGMQTFDQSLFDLYEADLISYDDALRNADSVNDLRLQIQLNSKKNTTAGSGGVLDGLSLTDYAPPEAEEK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=1141190 DQN52_RS07030 WP_003786168.1 1314558..1315742(-) (pilU) [Kingella kingae strain NCTC10529]
ATGCAAGTTATCATTCCACCCATCAACGAAGAAATGCGTAAATTTATCCATGGCTTGCTCACGCACATGGTCAATAACAA
AGGCTCGGATTTATTTATCACGGCTGGCTATCCACCTGCGATGAAATTGGACGGCAAATTAACCAAAATCACCGACAAGC
CTTTAACCGCCGACCACGCCATGCAAATTGCGCGTTCGATTATGGACGACAAGCAAGCCGAAGAATTTTTAACCACCAAC
GAATGCAATTTTGCGATTAGCTTGCCAGGCGTGTCGCGCTTTCGTATCAACGCCATGGTGCAACGTGGTGCAGCAGCTTT
GGTTTGCCGTGTGATTACCAGCGACATTCCCAAATTCGACAACATGAACTTACCGCCTGTGTTAAAACAAGTCGTGATGG
AAAAACGTGGTTTGGTGATTTTTGTCGGCGGTACAGGTTCGGGTAAATCCACATCGTTGGCGGCGATGATTGATTACCGC
AACGAAAACAGCTACGGTCATATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACAAAAACTGCATCATCAC
GCAACGCGAAGTGGGTGTGGACACCGAAAACTGGTTCGCCGCGCTCAAAAACACACTGCGCCAAGCACCCGATGTGATTT
TGATTGGTGAGATTCGTGACCGCGAAACGATGGACTACGCACTTGCCTTTGCCGAAACAGGGCACTTGTGTATGGCAACA
CTGCACGCGAATAACTCCAACCAGGCCCTTGACCGCATTATTAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGAA
CGACTTGTCGCTCAACTTAAAAGGCTTTATTTCGCAACGCCTTGTCCCCAAAAAACAAGGCAAAGGACGTGTGGCTGCGG
TAGAAATCCTGCTCAATTCGCCGTTGATTTCCGAATTGGTGTTGCATGGCGACATTCATGCGGTAAAAGAAATCATGGCA
AAATCGCGCGACATTGGTATGCAAACGTTCGACCAATCGCTGTTTGATTTGTATGAAGCCGATTTGATTTCGTATGACGA
TGCCTTGCGTAATGCTGACTCGGTAAACGATTTGCGCCTGCAAATTCAGTTAAACAGCAAGAAAAACACAACAGCTGGTT
CGGGCGGCGTATTGGACGGCTTGTCTTTGACCGATTACGCACCGCCAGAAGCAGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.689

89.086

0.523
  pilU Acinetobacter baylyi ADP1

58.239

89.34

0.52
  pilU Vibrio cholerae strain A1552

53.406

93.147

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.892

93.909

0.393
  pilT Legionella pneumophila strain Lp02

43.544

84.518

0.368
  pilT Legionella pneumophila strain ERS1305867

43.544

84.518

0.368

Multiple sequence alignment