Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQN92_RS16285 Genome accession   NZ_LS483377
Coordinates   3483973..3485706 (-) Length   577 a.a.
NCBI ID   WP_005414305.1    Uniprot ID   A0AA42QKG3
Organism   Stenotrophomonas maltophilia strain NCTC10258     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3478973..3490706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN92_RS16270 (NCTC10258_03226) sucC 3479273..3480442 (-) 1170 WP_111687599.1 ADP-forming succinate--CoA ligase subunit beta -
  DQN92_RS16275 (NCTC10258_03227) - 3480800..3482413 (+) 1614 WP_111687600.1 PAS domain-containing sensor histidine kinase -
  DQN92_RS16280 (NCTC10258_03228) pilR 3482474..3483847 (+) 1374 WP_100451078.1 sigma-54 dependent transcriptional regulator Regulator
  DQN92_RS16285 (NCTC10258_03229) pilB 3483973..3485706 (-) 1734 WP_005414305.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DQN92_RS16290 (NCTC10258_03230) - 3485929..3486336 (-) 408 WP_111687601.1 pilin -
  DQN92_RS16295 (NCTC10258_03231) - 3486485..3486913 (-) 429 WP_111687602.1 pilin -
  DQN92_RS16300 (NCTC10258_03232) pilC 3487266..3488525 (+) 1260 WP_111687603.1 type II secretion system F family protein Machinery gene
  DQN92_RS16305 (NCTC10258_03233) - 3488533..3489396 (+) 864 WP_049429911.1 A24 family peptidase -
  DQN92_RS16310 (NCTC10258_03234) coaE 3489408..3490019 (+) 612 WP_111687604.1 dephospho-CoA kinase -
  DQN92_RS20860 - 3490212..3490349 (-) 138 WP_172459932.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62317.25 Da        Isoelectric Point: 5.8722

>NTDB_id=1139193 DQN92_RS16285 WP_005414305.1 3483973..3485706(-) (pilB) [Stenotrophomonas maltophilia strain NCTC10258]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1139193 DQN92_RS16285 WP_005414305.1 3483973..3485706(-) (pilB) [Stenotrophomonas maltophilia strain NCTC10258]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCGCTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTTTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGATGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGTGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGATCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACAGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTTCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCTGCATTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.872

100

0.556
  pilB Acinetobacter baumannii D1279779

55

100

0.553
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio cholerae strain A1552

48.677

98.267

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447

Multiple sequence alignment