Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O987_RS04070 Genome accession   NZ_CP006704
Coordinates   903329..905071 (-) Length   580 a.a.
NCBI ID   WP_043370924.1    Uniprot ID   -
Organism   Comamonas testosteroni TK102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 898329..910071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O987_RS04040 (O987_04085) - 898846..899319 (+) 474 WP_200879588.1 NUDIX domain-containing protein -
  O987_RS04045 (O987_04090) - 899435..899647 (-) 213 WP_003058551.1 DNA gyrase inhibitor YacG -
  O987_RS04050 (O987_04095) zapD 899746..900501 (-) 756 WP_043370920.1 cell division protein ZapD -
  O987_RS04055 (O987_04100) coaE 900589..901215 (-) 627 WP_003058547.1 dephospho-CoA kinase -
  O987_RS04060 (O987_04105) - 901219..902088 (-) 870 WP_043370921.1 prepilin peptidase -
  O987_RS04065 (O987_04110) pilC 902088..903305 (-) 1218 WP_043370923.1 type II secretion system F family protein Machinery gene
  O987_RS04070 (O987_04115) pilB 903329..905071 (-) 1743 WP_043370924.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O987_RS04080 (O987_04125) - 905326..906255 (-) 930 WP_003058538.1 polyprenyl synthetase family protein -
  O987_RS04085 (O987_04130) rplU 906598..906909 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  O987_RS04090 (O987_04135) rpmA 906926..907183 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  O987_RS04095 (O987_04140) cgtA 907340..908455 (+) 1116 WP_003058532.1 Obg family GTPase CgtA -
  O987_RS04100 (O987_04145) proB 908566..909705 (+) 1140 WP_043370925.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63097.27 Da        Isoelectric Point: 5.0878

>NTDB_id=113594 O987_RS04070 WP_043370924.1 903329..905071(-) (pilB) [Comamonas testosteroni TK102]
MAAADNLQKNAPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDAQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQAGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRPDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIEAGYAEEELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=113594 O987_RS04070 WP_043370924.1 903329..905071(-) (pilB) [Comamonas testosteroni TK102]
ATGGCCGCTGCCGACAATCTGCAAAAGAACGCCCCTGCGCCCATTGCCATCCCTGGTCTAGGCAGAGCCCTGATTTCCGC
CGGCAAGGTCTCGCAGCAAGCAGCCGAAAACGCAGCCAAGAAAGCCGCCACAGGCAAGACTCCATTCATCACCGAACTGA
GCCAGTCCGGCGAAATCAGCGCACTGGAAATTGCCGAAACCATCTCCATGATGTTCAGCACACCGTTGCTGGACCTCAAT
GCCATCGACGCCCAGCAACTGCCACGCGAGCTGCTGGACCCGAAGATCAGCAACGCCTACCGCGTGCTGGCACTCAGCAA
GCGCGGCAACCGGCTCACCATTGCCACGGCAGACCCCACGCAGCAGGAAGCGGCCGAGCAGATCAAGTTCACAACCCAGT
TGCTGGTGGACTGGGTAGTTGTCGAGGCCGACAAGCTGCAAAAACTGGTAGAGCAGGCCGGCAAGAGCGTCAGCGAGTCT
CTGGACTCCTATTCCAGCTCCGGAGACTTTGACTTCGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CGTCGGCGCCGAGGTCGAGGATGCTCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGCTGCGCGAGATCGCCTCG
CCCCCGATTGCGATCAAGGACAAGCTGGCTTCCCGCATCAAGGTGATCTCGCGCCCGGACATCTCGGAGAAGCGCGTTCC
GCAGGACGGCCGCATGAAGCTCAAGGTCGGCCCCGACCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAGAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGATGCCCTCGGCTACGAACCCGAGGAA
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATCCTGGTCACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCACTCTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATCGCCACCGCCGAGGACCCTTCCGAAATCAACA
TGCCTGGCGTCAATCAGGTCAATGTGAACGAGAAGGCCGGCCTGACCTTTGCCGCTGCGCTCAAATCCTTTCTGCGTCAG
GACCCGGACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGATGCTCCGACCACACTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCTTCCAGCGTGATTCTGATCACCGCCCAGCGCCTGGCACGACGCCTGTGTGCTCAGTGCAAGGAACCCGCCGAC
ATACCGCACGACGCCCTGATCGAAGCCGGCTACGCTGAAGAGGAGCTCGACGGCAGCTGGGTCAGCTACAAGCCTGTGGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATCTATCAGGTGATGCCCATCAGCGAGGAGATTCAGCGCA
TCATCCTGCGTGACGGCAGTGCGCTGGAAATTGCTGAACAAGCCAAGGCCGAAGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-154)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.609

99.138

0.541
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.65

97.586

0.514
  pilB Legionella pneumophila strain ERS1305867

50.442

97.414

0.491
  pilB Vibrio cholerae strain A1552

46.898

94.483

0.443
  pilB Vibrio campbellii strain DS40M4

44.029

96.724

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

45.817

90.69

0.416
  pilF Thermus thermophilus HB27

38.021

99.31

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.246

98.276

0.376

Multiple sequence alignment