Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLWA5_RS06755 Genome accession   NZ_LR877307
Coordinates   1571793..1573517 (+) Length   574 a.a.
NCBI ID   WP_039525810.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1555230..1580317 1571793..1573517 within 0


Gene organization within MGE regions


Location: 1555230..1580317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWA5_RS06670 (X12_001327) - 1555230..1555592 (-) 363 WP_016904888.1 hypothetical protein -
  ACLWA5_RS06675 (X12_001328) - 1556082..1557362 (-) 1281 WP_080591431.1 retropepsin-like aspartic protease -
  ACLWA5_RS06680 (X12_001329) - 1557434..1557994 (-) 561 WP_102596783.1 hypothetical protein -
  ACLWA5_RS06685 (X12_001330) - 1558538..1558744 (+) 207 WP_374057769.1 ImmA/IrrE family metallo-endopeptidase -
  ACLWA5_RS06690 (X12_001331) - 1558729..1559241 (+) 513 WP_049805061.1 ImmA/IrrE family metallo-endopeptidase -
  ACLWA5_RS06695 (X12_001332) - 1559816..1560202 (+) 387 WP_026064907.1 hypothetical protein -
  ACLWA5_RS06700 (X12_001333) - 1560291..1560683 (+) 393 WP_023904915.1 H-NS family nucleoid-associated regulatory protein -
  ACLWA5_RS06705 (X12_001334) glgX 1561355..1563487 (-) 2133 WP_349812720.1 glycogen debranching protein GlgX -
  ACLWA5_RS06710 (X12_001335) rimK 1563977..1564882 (+) 906 WP_166767257.1 30S ribosomal protein S6--L-glutamate ligase -
  ACLWA5_RS06715 (X12_001336) - 1565109..1565579 (+) 471 WP_039525800.1 hypothetical protein -
  ACLWA5_RS06720 (X12_001337) - 1565611..1566288 (+) 678 WP_006448355.1 response regulator transcription factor -
  ACLWA5_RS06725 (X12_001338) - 1566281..1567615 (+) 1335 WP_016904089.1 sensor histidine kinase -
  ACLWA5_RS06730 (X12_001339) - 1567718..1567963 (-) 246 WP_374057770.1 SymE family type I addiction module toxin -
  ACLWA5_RS06735 (X12_001340) coaE 1567973..1568596 (-) 624 WP_039525803.1 dephospho-CoA kinase -
  ACLWA5_RS06740 (X12_001341) - 1568610..1569473 (-) 864 WP_016849610.1 prepilin peptidase -
  ACLWA5_RS06745 (X12_001342) pilC 1569480..1570739 (-) 1260 WP_039525808.1 type II secretion system F family protein Machinery gene
  ACLWA5_RS06750 - 1571084..1571491 (+) 408 WP_039529754.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACLWA5_RS06755 (X12_001344) pilB 1571793..1573517 (+) 1725 WP_039525810.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLWA5_RS06760 (X12_001345) pilR 1573731..1575125 (-) 1395 WP_053045681.1 sigma-54-dependent transcriptional regulator Regulator
  ACLWA5_RS06765 (X12_001346) - 1575318..1576931 (-) 1614 WP_349812721.1 sensor histidine kinase -
  ACLWA5_RS06770 (X12_001347) sucC 1577163..1578332 (+) 1170 WP_039519379.1 ADP-forming succinate--CoA ligase subunit beta -
  ACLWA5_RS06775 (X12_001348) sucD 1578357..1579232 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  ACLWA5_RS06780 (X12_001349) - 1579309..1579746 (-) 438 WP_180973917.1 hypothetical protein -
  ACLWA5_RS06785 (X12_001350) - 1579925..1580317 (+) 393 WP_026064065.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62495.58 Da        Isoelectric Point: 5.6021

>NTDB_id=1132571 ACLWA5_RS06755 WP_039525810.1 1571793..1573517(+) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
MNQNLVGITGIARRLVQDGVIDESVARSAMEQASTAKIPLPQWFSDKKLVSAPQLAAANAVEFGMPLMDVSVFDASQNAV
KLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGDDDD
DMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLN
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLE
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHAL
LAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALVKAAH
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1132571 ACLWA5_RS06755 WP_039525810.1 1571793..1573517(+) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
ATGAATCAGAATTTGGTTGGCATTACCGGTATCGCGCGGCGTCTAGTTCAAGATGGCGTTATAGACGAGTCTGTGGCCCG
CTCGGCAATGGAGCAGGCGTCAACAGCAAAGATTCCTTTGCCGCAGTGGTTTTCCGATAAGAAGCTGGTCTCAGCGCCTC
AACTTGCCGCTGCCAATGCAGTCGAGTTCGGTATGCCGCTGATGGACGTTTCCGTGTTTGACGCGAGCCAGAACGCCGTC
AAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGT
GAGCAATCCGACCCAGACGCGTGCGCTGGACGACATCAAGTTTCATACCAACCTGGTGGTAGAGCCTATCCTGGTCGACG
AGGATCAGATTCGTCGCACTTTGGAGCAGTGGCAGGCCAGCAATGCGTCGCTTGGCTCATCGCTCGGTGACGATGATGAC
GACATGGGAGACCTTGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGA
CGACACGCCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCACTTCGAGC
CGTATGAAGACGACTATCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCGGTGAAGCTGAAC
CAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAACGGGTGCCGCAGGACGGGCGCATAAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAAAGGTTGTGCTGC
GTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTATTCCTGGAG
GCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTTGTACACGGCACT
GGGTATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCCGTTGAAATTCGCTTGCCTGGCGTCAATCAGG
TGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCGCTGCGTTCGTTCTTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCTGTCCACGCT
GCATACCAACGACGCGCCACAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCTTACAACATTACTTCGTCGGTGA
CCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAGCATGCGTTG
CTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGAGTGTACCGA
AGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGTA
ACGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGCATCCGTGACCTGCGCCAGTCGGCGTTGGTCAAGGCGGCGCAC
GGTGTGACCAGCCTGGCCGAGATCAACCGAGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.955

0.551
  pilB Acinetobacter baylyi ADP1

55.282

98.955

0.547
  pilB Legionella pneumophila strain ERS1305867

52.197

99.129

0.517
  pilF Neisseria gonorrhoeae MS11

49.296

98.955

0.488
  pilB Vibrio cholerae strain A1552

48.27

100

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

45.993

100

0.46
  pilB Vibrio campbellii strain DS40M4

45.87

99.129

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

92.509

0.366
  pilF Thermus thermophilus HB27

40.192

90.592

0.364

Multiple sequence alignment