Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7A87_RS14980 Genome accession   NZ_LR861805
Coordinates   3524474..3526210 (-) Length   578 a.a.
NCBI ID   WP_119127591.1    Uniprot ID   -
Organism   Xanthomonas sp. CPBF 426 isolate     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3519474..3531210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A87_RS14965 (XSP_003006) sucC 3519583..3520752 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  H7A87_RS14970 (XSP_003007) - 3520975..3522588 (+) 1614 WP_104544483.1 HAMP domain-containing sensor histidine kinase -
  H7A87_RS14975 (XSP_003008) pilR 3522781..3524175 (+) 1395 WP_180496733.1 sigma-54 dependent transcriptional regulator Regulator
  H7A87_RS14980 (XSP_003009) pilB 3524474..3526210 (-) 1737 WP_119127591.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7A87_RS14985 (XSP_003010) galE 3526330..3527307 (-) 978 WP_119127589.1 UDP-glucose 4-epimerase GalE -
  H7A87_RS14990 (XSP_003011) - 3527403..3528947 (-) 1545 WP_119127587.1 NAD(P)/FAD-dependent oxidoreductase -
  H7A87_RS14995 (XSP_003012) - 3528949..3529410 (-) 462 WP_147421061.1 GtrA family protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62643.71 Da        Isoelectric Point: 5.4464

>NTDB_id=1132459 H7A87_RS14980 WP_119127591.1 3524474..3526210(-) (pilB) [Xanthomonas sp. CPBF 426 isolate]
MNAVNTANLVGITGLARRLVQDGALDEAAARDAMAKAAAARQPLPIWLTQNKVVGAAQLAAANAVEFGMPLMDVSVFDAS
QSAVKLVSEELLQKYQVLPLFRRGNRLFVGVSDPTQTRPLDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDDDMGDLDVSAGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRRSSLP
EHALLAEGFTAEQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1132459 H7A87_RS14980 WP_119127591.1 3524474..3526210(-) (pilB) [Xanthomonas sp. CPBF 426 isolate]
ATGAACGCCGTTAACACAGCTAACCTCGTCGGCATTACCGGCCTTGCCCGTCGCCTAGTCCAGGACGGCGCGCTGGATGA
AGCTGCAGCCCGCGATGCCATGGCAAAAGCGGCTGCAGCCAGGCAGCCACTGCCAATCTGGCTTACTCAGAACAAGGTCG
TCGGTGCTGCGCAACTGGCTGCCGCTAACGCAGTCGAGTTCGGCATGCCATTGATGGACGTGTCGGTATTCGACGCCAGC
CAGAGCGCCGTCAAGCTGGTCAGCGAGGAATTGCTTCAGAAGTACCAGGTGCTGCCATTGTTCAGGCGCGGTAACCGGTT
GTTCGTTGGGGTGAGTGACCCGACTCAGACACGTCCGTTGGACGACATCAAGTTCCATACCAATCTGGTGGTCGAGCCTA
TCCTGGTCGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGTCGCTTGGCTCATCGCTCGGT
GACGACGATGACGACATGGGGGATTTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATGCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAATTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTATCGGGTGCGCTTGCGCATCGACGGCCTGCTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGTATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAGGACCCGGTCGAAATCCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACGTTTGCGGCGGCGTTGCGCTCGTTCCTGCGCCAGGACCCT
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGTATTGCGCCGTACAACATCA
CCTCATCGGTGACGTTGGTGATTGCGCAGCGTCTGGCGCGACGGTTGTGCAACAACTGCAAGCGCAGATCGAGCCTGCCC
GAGCATGCATTGCTGGCGGAGGGGTTCACTGCAGAGCAGCTCGCTGCCGGTATCGAGCTGTACGAGGCAGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGCATCCGCGACCTGCGCCAGTCTGCGCTGGTC
AAGGCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTAACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.095

100

0.552
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio cholerae strain A1552

47.887

98.27

0.471
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.439

98.616

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

92.907

0.365
  pilF Thermus thermophilus HB27

39.36

91.869

0.362

Multiple sequence alignment