Detailed information NTG1132345

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: H1W81_RS06235
• Genome accession: NZ_LR824002
• Coordinates: 1200671..1201510 (-)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus isolate STH_CIRM_67
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1195671..1206510

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1W81_RS06220 (STHERMO_1388)	-	1196723..1197382 (-)	660	WP_011227158.1	response regulator transcription factor	-
H1W81_RS06225 (STHERMO_1389)	-	1197418..1198242 (-)	825	WP_011227157.1	TraX family protein	-
H1W81_RS06230 (STHERMO_1390)	topA	1198359..1200503 (-)	2145	WP_011227156.1	type I DNA topoisomerase	-
H1W81_RS06235 (STHERMO_1392)	dprA	1200671..1201510 (-)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
H1W81_RS06240 (STHERMO_1393)	-	1201578..1202498 (-)	921	WP_002950604.1	ROK family protein	-
H1W81_RS06245 (STHERMO_1394)	-	1202509..1203276 (-)	768	WP_002950603.1	ribonuclease HII	-
H1W81_RS06250 (STHERMO_1395)	ylqF	1203263..1204117 (-)	855	WP_002950602.1	ribosome biogenesis GTPase YlqF	-
H1W81_RS06255 (STHERMO_1396)	-	1204245..1204934 (-)	690	WP_011225885.1	XRE family transcriptional regulator	-
H1W81_RS06260 (STHERMO_1397)	-	1205029..1205544 (-)	516	WP_179971605.1	transcription repressor NadR	-
H1W81_RS06265 (STHERMO_1398)	-	1205568..1206095 (-)	528	WP_041827017.1	ECF transporter S component	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=1132345 H1W81_RS06235 WP_011225887.1 1200671..1201510(-) (dprA) [Streptococcus thermophilus isolate STH_CIRM_67]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=1132345 H1W81_RS06235 WP_011225887.1 1200671..1201510(-) (dprA) [Streptococcus thermophilus isolate STH_CIRM_67]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362