Detailed information NTG1132120

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: H1W86_RS04285
• Genome accession: NZ_LR822042
• Coordinates: 818304..819143 (+)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus isolate STH_CIRM_1358
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 813304..824143

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1W86_RS04255 (STHERMO_0905)	-	813720..814247 (+)	528	WP_014608250.1	ECF transporter S component	-
H1W86_RS04260 (STHERMO_0906)	-	814271..814786 (+)	516	WP_014608251.1	transcription repressor NadR	-
H1W86_RS04265 (STHERMO_0907)	-	814881..815570 (+)	690	WP_014608252.1	XRE family transcriptional regulator	-
H1W86_RS04270 (STHERMO_0908)	ylqF	815698..816552 (+)	855	WP_180479697.1	ribosome biogenesis GTPase YlqF	-
H1W86_RS04275 (STHERMO_0909)	-	816539..817306 (+)	768	WP_180479699.1	ribonuclease HII	-
H1W86_RS04280 (STHERMO_0910)	-	817317..818237 (+)	921	WP_014608255.1	ROK family protein	-
H1W86_RS04285 (STHERMO_0911)	dprA	818304..819143 (+)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
H1W86_RS04290 (STHERMO_0912)	topA	819311..821455 (+)	2145	WP_064355758.1	type I DNA topoisomerase	-
H1W86_RS04295 (STHERMO_0913)	-	821572..822396 (+)	825	WP_014608257.1	TraX family protein	-
H1W86_RS04300 (STHERMO_0914)	-	822432..823091 (+)	660	WP_011227158.1	response regulator transcription factor	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=1132120 H1W86_RS04285 WP_011225887.1 818304..819143(+) (dprA) [Streptococcus thermophilus isolate STH_CIRM_1358]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=1132120 H1W86_RS04285 WP_011225887.1 818304..819143(+) (dprA) [Streptococcus thermophilus isolate STH_CIRM_1358]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTAACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCGGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362