Detailed information    

insolico Bioinformatically predicted

Overview


Name   comYF   Type   Machinery gene
Locus tag   H1W95_RS08835 Genome accession   NZ_LR822030
Coordinates   1720741..1721178 (-) Length   145 a.a.
NCBI ID   WP_011681686.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_1046     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1715741..1726178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W95_RS08800 (STHERMO_1993) - 1715805..1716467 (-) 663 WP_084826165.1 type II CAAX endopeptidase family protein -
  H1W95_RS08805 (STHERMO_1994) - 1716564..1717007 (-) 444 WP_041829335.1 hypothetical protein -
  H1W95_RS10365 (STHERMO_1995) - 1717081..1717419 (-) 339 WP_228024408.1 CPBP family intramembrane glutamic endopeptidase -
  H1W95_RS10370 (STHERMO_1996) - 1717463..1717738 (-) 276 WP_084830830.1 CAAX protease -
  H1W95_RS08815 (STHERMO_1997) - 1717750..1717947 (-) 198 WP_084826167.1 helix-turn-helix transcriptional regulator -
  H1W95_RS08820 (STHERMO_1998) - 1718196..1719389 (-) 1194 WP_084826168.1 acetate kinase -
  H1W95_RS08825 (STHERMO_1999) comYH 1719445..1720401 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H1W95_RS08830 (STHERMO_2000) comGG 1720446..1720763 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  H1W95_RS08835 (STHERMO_2001) comYF 1720741..1721178 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W95_RS08840 (STHERMO_2002) comGE 1721162..1721455 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  H1W95_RS08845 (STHERMO_2003) comYD 1721427..1721855 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1W95_RS08850 (STHERMO_2004) comYC 1721815..1722141 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W95_RS08855 (STHERMO_2005) comYB 1722138..1723238 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W95_RS08860 (STHERMO_2006) comGA/cglA/cilD 1723120..1724061 (-) 942 WP_180483385.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W95_RS08865 (STHERMO_2007) - 1724142..1724504 (-) 363 WP_014608746.1 DUF1033 family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16375.85 Da        Isoelectric Point: 8.4500

>NTDB_id=1131488 H1W95_RS08835 WP_011681686.1 1720741..1721178(-) (comYF) [Streptococcus thermophilus isolate STH_CIRM_1046]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1131488 H1W95_RS08835 WP_011681686.1 1720741..1721178(-) (comYF) [Streptococcus thermophilus isolate STH_CIRM_1046]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566

  comYF Streptococcus mutans UA159

57.447

97.241

0.559

  comGF/cglF Streptococcus pneumoniae D39

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae Rx1

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae R6

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae TIGR4

49.286

96.552

0.476

  comGF/cglF Streptococcus mitis NCTC 12261

47.518

97.241

0.462

  comGF/cglF Streptococcus mitis SK321

46.809

97.241

0.455

  comGF Lactococcus lactis subsp. cremoris KW2

45.324

95.862

0.434


Multiple sequence alignment