Detailed information NTG1130908

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: H0506_RS04355
• Genome accession: NZ_LR822015
• Coordinates: 825428..826267 (+)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus isolate STH_CIRM_65
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 820428..831267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0506_RS04325 (STHERMO_0929)	-	820843..821370 (+)	528	WP_041827017.1	ECF transporter S component	-
H0506_RS04330 (STHERMO_0930)	-	821394..821909 (+)	516	WP_179971605.1	transcription repressor NadR	-
H0506_RS04335 (STHERMO_0931)	-	822004..822693 (+)	690	WP_011225885.1	helix-turn-helix transcriptional regulator	-
H0506_RS04340 (STHERMO_0932)	ylqF	822821..823675 (+)	855	WP_002950602.1	ribosome biogenesis GTPase YlqF	-
H0506_RS04345 (STHERMO_0933)	-	823662..824429 (+)	768	WP_002950603.1	ribonuclease HII	-
H0506_RS04350 (STHERMO_0934)	-	824440..825360 (+)	921	WP_002950604.1	ROK family protein	-
H0506_RS04355 (STHERMO_0935)	dprA	825428..826267 (+)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
H0506_RS04360 (STHERMO_0937)	topA	826435..828579 (+)	2145	WP_011227156.1	type I DNA topoisomerase	-
H0506_RS04365 (STHERMO_0938)	-	828696..829520 (+)	825	WP_011227157.1	TraX family protein	-
H0506_RS04370 (STHERMO_0939)	-	829556..830215 (+)	660	WP_011227158.1	response regulator transcription factor	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=1130908 H0506_RS04355 WP_011225887.1 825428..826267(+) (dprA) [Streptococcus thermophilus isolate STH_CIRM_65]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=1130908 H0506_RS04355 WP_011225887.1 825428..826267(+) (dprA) [Streptococcus thermophilus isolate STH_CIRM_65]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362