Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   H0504_RS01900 Genome accession   NZ_LR822011
Coordinates   362374..363069 (+) Length   231 a.a.
NCBI ID   WP_011226400.1    Uniprot ID   Q5M374
Organism   Streptococcus thermophilus isolate STH_CIRM_23     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 357374..368069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0504_RS01880 (STHERMO_0400) - 359359..359628 (-) 270 WP_011226404.1 GIY-YIG nuclease family protein -
  H0504_RS01885 (STHERMO_0401) - 359632..360396 (-) 765 WP_011226403.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  H0504_RS10075 - 360446..360895 (-) 450 WP_311316658.1 hypothetical protein -
  H0504_RS09465 (STHERMO_0403) - 360778..361311 (-) 534 WP_011226401.1 polysaccharide deacetylase family protein -
  H0504_RS01895 (STHERMO_0404) - 361511..362266 (+) 756 WP_002948862.1 lysophospholipid acyltransferase family protein -
  H0504_RS01900 (STHERMO_0405) comEA 362374..363069 (+) 696 WP_011226400.1 helix-hairpin-helix domain-containing protein Machinery gene
  H0504_RS01905 (STHERMO_0406) - 363059..365299 (+) 2241 WP_011227470.1 DNA internalization-related competence protein ComEC/Rec2 -
  H0504_RS01910 (STHERMO_0407) - 365385..366647 (+) 1263 WP_022096991.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  H0504_RS01915 (STHERMO_0408) - 366713..367258 (+) 546 WP_041828309.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 24466.40 Da        Isoelectric Point: 4.6300

>NTDB_id=1130655 H0504_RS01900 WP_011226400.1 362374..363069(+) (comEA) [Streptococcus thermophilus isolate STH_CIRM_23]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKAGARVTDVIQEAGGITEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTGTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRAEDIIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide


Download         Length: 696 bp        

>NTDB_id=1130655 H0504_RS01900 WP_011226400.1 362374..363069(+) (comEA) [Streptococcus thermophilus isolate STH_CIRM_23]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCAGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAAACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGCAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATTACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAGGTACTGGTCAGGTCTCTGACAAAGGG
GGGCAGGCTGTATCTAAGGATGGCAAAATTAACTTAAATACAGCGACCTCAGAACAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGGCAGAGGATATCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(85-138)

Helix-hairpin-helix motif (HHH_3)

(167-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M374

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

99.134

100

0.991
  comEA/celA/cilE Streptococcus mitis SK321

41.048

99.134

0.407
  comEA/celA/cilE Streptococcus pneumoniae D39

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae R6

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae Rx1

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.158

98.701

0.377

Multiple sequence alignment