Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1X59_RS02810 Genome accession   NZ_LR813086
Coordinates   575685..577430 (-) Length   581 a.a.
NCBI ID   WP_234687621.1    Uniprot ID   A0AA35D8W7
Organism   Comamonas aquatica isolate BB1454     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570685..582430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1X59_RS02780 (TML_00544) - 571263..571736 (+) 474 WP_234687617.1 NUDIX domain-containing protein -
  L1X59_RS02785 (TML_00545) - 571839..572048 (-) 210 WP_234687618.1 DNA gyrase inhibitor YacG -
  L1X59_RS02790 (TML_00546) zapD 572076..572831 (-) 756 WP_210930172.1 cell division protein ZapD -
  L1X59_RS02795 (TML_00547) coaE 572926..573534 (-) 609 WP_234687619.1 dephospho-CoA kinase -
  L1X59_RS02800 (TML_00548) - 573537..574410 (-) 874 Protein_538 prepilin peptidase -
  L1X59_RS02805 (TML_00549) pilC 574410..575633 (-) 1224 WP_234687620.1 type II secretion system F family protein Machinery gene
  L1X59_RS02810 (TML_00550) pilB 575685..577430 (-) 1746 WP_234687621.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L1X59_RS02815 (TML_00551) - 577589..578518 (-) 930 WP_234688389.1 polyprenyl synthetase family protein -
  L1X59_RS02820 (TML_00552) rplU 578729..579040 (+) 312 WP_042417032.1 50S ribosomal protein L21 -
  L1X59_RS02825 (TML_00553) rpmA 579059..579319 (+) 261 WP_042416998.1 50S ribosomal protein L27 -
  L1X59_RS02830 (TML_00554) cgtA 579483..580556 (+) 1074 WP_234687622.1 Obg family GTPase CgtA -
  L1X59_RS02835 (TML_00555) proB 580637..581773 (+) 1137 WP_234687623.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63291.54 Da        Isoelectric Point: 6.3015

>NTDB_id=1130403 L1X59_RS02810 WP_234687621.1 575685..577430(-) (pilB) [Comamonas aquatica isolate BB1454]
MNAASTASSIAQSSSNTALAGLARALVSAGQLTETVALETARKAHASKNSFVAQLVASGHMPAAELAFQIAQIFRTPLLD
LDTIDPQRLPRDLLDAKLCSMHKVLPLLKRGNRLVVATADPTDKDAAEKIKFTAQMGVDWVIVECDKLQRLVEATTKSAS
EALDAFNSDADFQFDEVVDAPAPDDKDSNASEVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHSYRVRFRIDGELREIA
SPPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPE
EKGRLLDAIQRPYGMILVTGPTGSGKTVSLYSCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFATALRSFLR
QDPDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPH
DVPHETLVEAGYEEHQLNGSWVPYKAVGCSACNNGYKGRVGIYQVMPITEAIQQIILRDGSALEIAAQARSEGVKSLRES
GLDKVRQGITSLEEVLAVTNL

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=1130403 L1X59_RS02810 WP_234687621.1 575685..577430(-) (pilB) [Comamonas aquatica isolate BB1454]
ATGAACGCCGCATCCACTGCATCCTCGATCGCCCAGTCCTCCTCGAACACGGCCCTGGCCGGGCTGGCCCGGGCCCTGGT
CAGTGCAGGCCAGCTCACGGAAACGGTCGCGCTGGAGACGGCCCGCAAGGCCCACGCCAGCAAGAACAGCTTCGTGGCAC
AGCTGGTGGCATCCGGGCACATGCCTGCGGCGGAACTGGCCTTCCAGATCGCACAGATTTTTCGCACGCCGCTGCTGGAC
CTGGACACCATCGATCCCCAGCGCCTGCCCAGGGATTTGCTGGACGCCAAGCTGTGCAGCATGCACAAGGTGCTGCCCCT
GCTCAAGCGCGGCAACCGCCTGGTGGTGGCCACAGCAGACCCCACCGACAAGGACGCCGCGGAGAAGATCAAATTCACCG
CCCAGATGGGGGTGGACTGGGTCATCGTCGAGTGCGACAAACTGCAGCGGCTGGTCGAAGCCACCACCAAGAGTGCCTCT
GAGGCCCTGGACGCTTTCAATTCCGATGCCGACTTCCAGTTCGACGAAGTGGTGGATGCGCCCGCCCCCGACGACAAGGA
CAGCAATGCATCCGAGGTCGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATTGACGCGTTCAACATGCGGG
CCTCGGACCTGCACTTTGAGCCCTATGAACACAGCTACCGCGTGCGCTTCCGGATCGATGGCGAACTGCGGGAGATCGCA
AGCCCGCCCATCGCCATCAAGGAAAAACTGGCCTCGCGCATCAAGGTGATCTCGCGACTGGACATCTCGGAAAAACGCGT
TCCCCAGGATGGGCGCATGAAGCTCAAGGTCGGTGCTGACCGCGTCATCGACTTCCGTGTGTCCACCTTGCCCACGCTGT
TCGGCGAGAAGATCGTGATCCGTATTCTGGACCCCAGCAGCGCCAGGATGGGCATCGATGCCCTGGGCTACGAGCCGGAG
GAAAAGGGACGTCTGCTGGATGCCATCCAGCGGCCCTACGGCATGATCCTGGTCACAGGGCCCACCGGCTCGGGCAAGAC
CGTGTCGCTGTACAGCTGCCTGAACCTGCTGAACAAACCCGGGGTCAACATCTCCACGGCCGAAGATCCCTCGGAAATCA
ACATGCCCGGCGTCAACCAGGTCAATGTGAATGAAAAGGCCGGACTGACTTTCGCCACCGCCCTGCGCTCCTTCCTGCGC
CAGGACCCGGATGTCATCATGGTCGGTGAAATCCGTGACCTGGAAACGGCCGACATCGCCATCAAGGCCGCGCAGACGGG
GCACTTGGTGCTATCCACCCTGCACACCAACGATGCGCCCACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCGT
TCAACATCGCCTCCAGCGTGATCCTGATCACGGCCCAGCGTCTGGCGCGCCGCTTGTGCCCACAATGCAAGCAGCCGCAT
GATGTGCCACACGAAACCCTGGTCGAAGCCGGTTACGAGGAACACCAGCTGAATGGCTCCTGGGTCCCCTACAAGGCTGT
GGGCTGCAGTGCCTGCAACAATGGTTACAAAGGCCGCGTGGGCATCTACCAGGTGATGCCGATCACCGAAGCAATCCAGC
AAATCATCCTGCGCGACGGCAGTGCCCTGGAGATTGCCGCCCAGGCCAGGAGCGAAGGCGTGAAATCGCTGCGCGAATCC
GGATTGGACAAAGTCCGGCAAGGCATCACTTCGCTGGAAGAGGTGCTGGCCGTCACCAATTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-155)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.3

97.418

0.539
  pilB Acinetobacter baylyi ADP1

55.496

97.074

0.539
  pilF Neisseria gonorrhoeae MS11

52.043

96.902

0.504
  pilB Legionella pneumophila strain ERS1305867

50.611

98.623

0.499
  pilB Vibrio cholerae strain A1552

47.016

95.181

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.893

96.386

0.442
  pilB Vibrio campbellii strain DS40M4

45.811

96.558

0.442
  pilF Thermus thermophilus HB27

41.144

93.287

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

91.738

0.363

Multiple sequence alignment