Detailed information NTG0113023

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSYRH_RS11770
• Genome accession: NZ_CP006256
• Coordinates: 2659929..2661098 (+)
• Length: 389 a.a.
• NCBI ID: WP_003435515.1
• Uniprot ID: A0A8T8LR20
• Organism: Pseudomonas syringae pv. syringae HS191
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2654929..2666098

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYRH_RS11755 (PsyrH_11985)	-	2655318..2657645 (+)	2328	WP_046719325.1	ATP-dependent DNA helicase	-
PSYRH_RS11760 (PsyrH_11990)	pgm	2657809..2659455 (+)	1647	WP_046719326.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSYRH_RS11765 (PsyrH_11995)	-	2659530..2659805 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
PSYRH_RS11770 (PsyrH_12000)	pilU	2659929..2661098 (+)	1170	WP_003435515.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSYRH_RS11775 (PsyrH_12005)	-	2661149..2662726 (-)	1578	WP_046719327.1	ABC transporter ATP-binding protein	-
PSYRH_RS11780 (PsyrH_12010)	-	2662728..2663750 (-)	1023	WP_024663647.1	ABC transporter permease	-
PSYRH_RS11785 (PsyrH_12015)	-	2663750..2664811 (-)	1062	WP_024663646.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42649.71 Da
• Isoelectric Point: 5.8676

>NTDB_id=113023 PSYRH_RS11770 WP_003435515.1 2659929..2661098(+) (pilU) [Pseudomonas syringae pv. syringae HS191]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=113023 PSYRH_RS11770 WP_003435515.1 2659929..2661098(+) (pilU) [Pseudomonas syringae pv. syringae HS191]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTACCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGAGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCCGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTACTGCTTGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACCGGTT
CGGGCAAGTCGACCTCGTTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGTCGTGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGTGAAGAAGAGGCCCTCAAGAACGCCGATTCACAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGTGAAGGCGGCGCCGGAACACTGACCACACCGCCACCGGCTCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGAGCCGGGTCCGCAAGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.331	99.486	0.56
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	50.829	93.059	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362