Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSYRH_RS02000 Genome accession   NZ_CP006256
Coordinates   422010..424115 (+) Length   701 a.a.
NCBI ID   WP_046718849.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae HS191     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417010..429115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRH_RS01980 (PsyrH_02025) pilM 419173..420237 (+) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  PSYRH_RS01985 (PsyrH_02030) - 420237..420818 (+) 582 WP_003316651.1 PilN domain-containing protein -
  PSYRH_RS01990 (PsyrH_02035) pilO 420815..421438 (+) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  PSYRH_RS01995 (PsyrH_02040) pilP 421435..421962 (+) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  PSYRH_RS02000 (PsyrH_02045) pilQ 422010..424115 (+) 2106 WP_046718849.1 type IV pilus secretin PilQ Machinery gene
  PSYRH_RS02005 (PsyrH_02050) aroK 424120..424638 (+) 519 WP_003341387.1 shikimate kinase AroK -
  PSYRH_RS02010 (PsyrH_02055) aroB 424754..425857 (+) 1104 WP_003392312.1 3-dehydroquinate synthase -
  PSYRH_RS02015 (PsyrH_02060) - 425868..427484 (+) 1617 WP_024662384.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75436.81 Da        Isoelectric Point: 5.1826

>NTDB_id=113018 PSYRH_RS02000 WP_046718849.1 422010..424115(+) (pilQ) [Pseudomonas syringae pv. syringae HS191]
MTRILSIIGVSLCIALLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGSGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=113018 PSYRH_RS02000 WP_046718849.1 422010..424115(+) (pilQ) [Pseudomonas syringae pv. syringae HS191]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGTTGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGCGATCGCATCGAGCTGAAGCTGGCATTCGATGCCCCGGTGCCTGCACCGCGCG
GTTACACCACGTCGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAT
CTGGGCAGCGGCAATGCGCGCAGTGTGGTTGTGGTTCAGGCTCAGGACCGCACTCGGGTGATCATCAACCTGACCACGCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCTGGCGCCGCGCAAGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTCGCGGTGCCGCCGCCCACGCCGGCATCCGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACGATCAGCGTGCGTCCATTGAC
CAACGAGGACGTCGACCGCCGCGCCGCTGACAAACCGGCGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGTGGTATC
ACCCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGCTCGATCACGGTGGACGACCGGACCAATAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAACTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTTCGCTGGGGCGGCTCTACGAACACCAGCGGTAACAGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTT
CGTGGACCTGGGCGCAGCCGGCGCCACCTCGGGTATCGGCCTTGGTTTCGTGACCAACAACACGTTGCTGGACCTTGAAC
TGAGTGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAAGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGTGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCTGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.917

100

0.749
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.405
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.061

98.573

0.375
  pilQ Legionella pneumophila strain Lp02

38.026

98.288

0.374

Multiple sequence alignment