Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AACL56_RS23135 Genome accession   NZ_LR743507
Coordinates   4895580..4897313 (+) Length   577 a.a.
NCBI ID   WP_339092131.1    Uniprot ID   -
Organism   Variovorax paradoxus isolate vvax     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4890580..4902313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACL56_RS23105 (VVAX_04610) proB 4891191..4892330 (-) 1140 WP_339092127.1 glutamate 5-kinase -
  AACL56_RS23110 (VVAX_04611) cgtA 4892420..4893496 (-) 1077 WP_339092128.1 Obg family GTPase CgtA -
  AACL56_RS23115 (VVAX_04612) rpmA 4893568..4893828 (-) 261 WP_081267930.1 50S ribosomal protein L27 -
  AACL56_RS23120 (VVAX_04613) rplU 4893841..4894152 (-) 312 WP_057592097.1 50S ribosomal protein L21 -
  AACL56_RS23125 (VVAX_04614) - 4894344..4895336 (+) 993 WP_339092130.1 polyprenyl synthetase family protein -
  AACL56_RS23135 (VVAX_04617) pilB 4895580..4897313 (+) 1734 WP_339092131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AACL56_RS23140 (VVAX_04618) pilC 4897341..4898570 (+) 1230 WP_339092132.1 type II secretion system F family protein Machinery gene
  AACL56_RS23145 (VVAX_04619) - 4898570..4899535 (+) 966 WP_339092133.1 A24 family peptidase -
  AACL56_RS23150 (VVAX_04620) coaE 4899544..4900152 (+) 609 WP_339092929.1 dephospho-CoA kinase -
  AACL56_RS23155 (VVAX_04621) - 4900222..4901715 (+) 1494 WP_339092134.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62466.70 Da        Isoelectric Point: 5.4417

>NTDB_id=1129795 AACL56_RS23135 WP_339092131.1 4895580..4897313(+) (pilB) [Variovorax paradoxus isolate vvax]
MAAAELPVKEPTQIALPGLARALVSAGKLPAKSAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSTAFGAPLLDLDA
IDPQRLPKDLLDAKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEATAASAAETI
NSIVGSEFEFDDVSADTASESSEQAIAEVEDAPVVRFLHKMLLDAISMRASDIHFEPYEHQYRVRFRIDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTTCKAPTDIPR
EALLEAGFQEEMLDGSWKPYRPVGCAACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIATQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1129795 AACL56_RS23135 WP_339092131.1 4895580..4897313(+) (pilB) [Variovorax paradoxus isolate vvax]
ATGGCCGCTGCCGAACTTCCCGTCAAAGAACCCACGCAGATTGCCCTTCCCGGGCTAGCGCGCGCCCTGGTTTCGGCCGG
CAAACTTCCTGCCAAGTCCGCGGAGGACATCTATCAGAAGTCGCTGAGCGGCCGTACCAGTTTCATCGCCGAACTGACGG
GTTCCGGCGCCGTGTCGGCCGCCGATCTTGCGCACACGCTGTCGACAGCCTTCGGCGCCCCGCTGCTCGATCTCGACGCG
ATTGACCCGCAGCGCCTACCCAAGGATCTTCTCGACGCCAAGCTGTGCCATGCATACCGCATCGTTGTGCTCAGCAAGCG
CAACAACCGTCTCATTGTTGCGACAGCTGACCCTTCCGACCAGCAGGCGGCAGAGAAGATCAAGTTTGCGTCTCAGATGG
GCGTTGACTGGGTCATCGCGGAGTACGACAAGCTGTCGCGCATGATCGAGGCCACCGCGGCGAGTGCGGCGGAGACCATC
AACAGCATCGTCGGCAGCGAGTTCGAGTTCGACGACGTTTCTGCAGACACCGCGAGCGAATCCAGTGAACAGGCAATCGC
GGAAGTCGAAGATGCACCGGTCGTACGCTTCCTGCACAAGATGCTGCTCGACGCGATCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCTTACGAACACCAGTACCGCGTGCGTTTCCGGATCGACGGAGAACTGCGTGAAATCGCGAGCCCGCCCACG
GTCATCAAGGACAAGCTGGCATCACGCATCAAGGTGATTTCACGTCTCGATATTTCGGAAAAGCGCGTCCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTCATCGACTTCCGTGTAAGCACTTTACCGACGTTGTTCGGCGAGAAGA
TCGTCATTCGTATTCTCGACCCCAGCAGTGCACGCCTGGGCATTGACGCGCTGGGCTACGACGCGGACGAAAAGGAGCGG
CTGCTTCATGCGATCGGCCGTCCATATGGCATGGTGCTTGTTACCGGCCCAACGGGCTCGGGCAAGACGGTGTCGCTGTA
CACCTGCCTGAACCTTCTGAACCAACCGGGCGTGAATATCGCGACGGCGGAAGACCCGTCCGAAATCAACTTGCCCGGCG
TGAACCAGGTCAACGTCAACGAGAAGGCAGGACTGACCTTTGCCGCCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGATCTGGAAACCGCCGACATCTCGATCAAGGCTGCCCAGACCGGTCACCTGGTCCT
GTCCACGCTGCACACCAACGACGCCCCCACGACGCTCACGCGGATGCGCAACATGGGGATCGCACCGTTCAACATCGCAT
CGAGCGTCATCCTGATCACGGCGCAACGGCTCGCACGGCGCCTGTGCACGACGTGCAAGGCTCCCACCGACATACCGCGG
GAAGCCCTGCTGGAGGCGGGCTTCCAAGAAGAAATGCTCGACGGTAGCTGGAAGCCATATCGCCCGGTCGGCTGCGCAGC
ATGCAGCGGCGGCTACAAGGGCCGCGTCGGCATTTACCAAGTGATGCCGATTTCCGAGGCCATCCAGGAAATCATCCTGC
GCGACGGCAGCGCCCTCGACATCGCCACGCAATCGGAAGCCGAAGGCGTGCGCTCGCTACGGCAGTCCGGCCTGCGCAAG
GTCATGCAAGGCCTCACTTCGCTCGAAGAAGTGGTGGCCTGCACCAACTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.08

97.227

0.536
  pilB Acinetobacter baylyi ADP1

53.616

98.267

0.527
  pilF Neisseria gonorrhoeae MS11

52.847

97.4

0.515
  pilB Legionella pneumophila strain ERS1305867

49.464

97.054

0.48
  pilB Vibrio cholerae strain A1552

47.698

94.107

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

97.747

0.449
  pilB Vibrio campbellii strain DS40M4

45.293

97.574

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.884

94.107

0.385
  pilF Thermus thermophilus HB27

38.448

98.267

0.378

Multiple sequence alignment