Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5P2_RS05950 Genome accession   NZ_LR594666
Coordinates   1235178..1236911 (-) Length   577 a.a.
NCBI ID   WP_162566228.1    Uniprot ID   A0A8B6LHI9
Organism   Variovorax sp. SRS16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230178..1241911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P2_RS05930 (G3W92_RS05930) - 1230889..1232385 (-) 1497 WP_162566224.1 MBOAT family protein -
  E5P2_RS05935 (G3W92_RS05935) coaE 1232467..1233066 (-) 600 WP_162566225.1 dephospho-CoA kinase -
  E5P2_RS05940 (G3W92_RS05940) pilD 1233080..1233934 (-) 855 WP_162566226.1 A24 family peptidase Machinery gene
  E5P2_RS05945 (G3W92_RS05945) pilC 1233934..1235157 (-) 1224 WP_162566227.1 type II secretion system F family protein Machinery gene
  E5P2_RS05950 (G3W92_RS05950) pilB 1235178..1236911 (-) 1734 WP_162566228.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5P2_RS05960 (G3W92_RS05960) - 1237145..1238137 (-) 993 WP_162566229.1 polyprenyl synthetase family protein -
  E5P2_RS05965 (G3W92_RS05965) rplU 1238324..1238635 (+) 312 WP_162566230.1 50S ribosomal protein L21 -
  E5P2_RS05970 (G3W92_RS05970) rpmA 1238648..1238905 (+) 258 WP_162566231.1 50S ribosomal protein L27 -
  E5P2_RS05975 (G3W92_RS05975) obgE 1238974..1240029 (+) 1056 WP_162566232.1 GTPase ObgE -
  E5P2_RS05980 (G3W92_RS05980) proB 1240031..1241182 (+) 1152 WP_162566233.1 glutamate 5-kinase -
  E5P2_RS05985 (G3W92_RS05985) - 1241187..1241768 (-) 582 WP_162566234.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62500.81 Da        Isoelectric Point: 5.6281

>NTDB_id=1128205 E5P2_RS05950 WP_162566228.1 1235178..1236911(-) (pilB) [Variovorax sp. SRS16]
MAAAEPLVKESSPIALPGLARALISAGKLPAKVAEDIYQKSLSGRSSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDPQRLPKDLLDPKLCTAYRVVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
DNIIGAEFEFDEVSSDTSSEANDSAAAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRIPQDGRMKLKIGPERVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLTAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRVPADVPA
QALLDAGFKTQDLDNSWKPYRPVGCSACNSGYKGRVGIYQVMPITEEIQKIILRDGSALDIAKQAEAEGVRSLRQSGLKK
VMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1128205 E5P2_RS05950 WP_162566228.1 1235178..1236911(-) (pilB) [Variovorax sp. SRS16]
ATGGCCGCTGCCGAACCCCTTGTCAAAGAAAGCTCGCCGATCGCCCTTCCAGGGCTCGCGCGTGCGTTGATCTCGGCCGG
AAAGCTGCCGGCCAAGGTCGCCGAGGACATCTATCAGAAGTCGCTCAGCGGCCGCAGCAGCTTCATTGCTGAGCTCACAG
GCACCGGCGCCGTGTCGGCCGCCGACCTGGCTCACACGCTGTCCAGCGCCTTCGGCGCCCCCTTGCTGGACCTGGACGCC
ATCGACCCGCAGCGGCTGCCGAAGGACCTTCTCGACCCCAAGCTGTGCACGGCCTACCGGGTGGTCGTCCTCAGCAAGCG
CAACAACCGTCTCATTGTCGCAACAGCTGACCCGTCCGATCAGCAGGCCGCCGAAAAGATCAAGTTTGCGTCGCAGATGG
GCGTGGACTGGGTCATTGCCGAGTACGACAAGCTCTCGCGCATGATCGAAGCGGCCGCCGTGAGCGCGGCCGAAACCATC
GACAACATCATCGGCGCCGAGTTCGAGTTCGACGAGGTGTCCTCGGACACTTCGAGCGAGGCCAACGATTCCGCGGCGGC
CGAAGTCGAAGATGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCCTTCAGCATGCGCGCGTCGGACATCC
ACTTCGAGCCCTACGAACATCAATACCGCGTGCGCTTTCGCGTCGATGGCGAATTGCGCGAGATCGCAAGCCCGCCCACG
GTCATCAAGGACAAGCTGGCCTCCCGCATCAAGGTGATTTCGCGCCTGGATATTTCCGAGAAGCGGATTCCGCAGGACGG
CCGGATGAAGCTCAAGATCGGTCCGGAGCGTGTGATCGATTTTCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAAAAGA
TCGTCATCCGTATCCTCGACCCGAGCAGCGCACGCCTGGGCATTGATGCGCTGGGCTACGACGCGGACGAAAAGGAGCGC
CTGCTCACCGCGATCGGCCGTCCTTACGGCATGGTGCTGGTCACCGGGCCCACGGGTTCCGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTCAACAAGCCCGGCGTCAACATCGCCACGGCCGAAGACCCGTCCGAAATCAACCTTCCCGGCG
TGAACCAGGTCAACGTCAACGAAAAGGCGGGACTGACCTTCGCCACCGCGCTGCGCGCGTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACGGCCGACATCTCGATCAAAGCCGCGCAGACGGGCCACCTGGTTCT
CTCCACGCTGCACACCAACGACGCTCCGACGACGCTGACGCGGATGCGCAACATGGGTATCGCGCCCTTCAACATCGCGT
CGAGCGTGATCCTGATCACCGCGCAGCGGCTCGCACGCCGTCTGTGCACGGTCTGCCGCGTGCCGGCAGACGTGCCCGCG
CAGGCCTTGCTCGATGCCGGCTTCAAGACGCAGGACCTGGACAACTCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCGGC
CTGCAACAGCGGCTACAAGGGGCGTGTCGGCATCTACCAGGTGATGCCGATCACGGAAGAAATCCAGAAGATCATCCTGC
GCGACGGCAGCGCCCTCGACATCGCCAAGCAGGCCGAGGCCGAGGGCGTTCGCTCGCTGCGGCAATCTGGACTGAAGAAG
GTCATGCAAGGACTGACTTCACTTGAAGAAGTGCTGGCAGTCACCAACGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B6LHI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.401

98.44

0.536
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilF Neisseria gonorrhoeae MS11

52.931

97.574

0.516
  pilB Legionella pneumophila strain ERS1305867

50.177

98.094

0.492
  pilB Vibrio campbellii strain DS40M4

45.439

98.787

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

48.099

91.161

0.438
  pilB Vibrio cholerae strain A1552

48.738

89.255

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.585

94.801

0.385
  pilF Thermus thermophilus HB27

38.144

100

0.385

Multiple sequence alignment