Detailed information NTG1128203

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E5P2_RS05940
• Genome accession: NZ_LR594666
• Coordinates: 1233080..1233934 (-)
• Length: 284 a.a.
• NCBI ID: WP_162566226.1
• Uniprot ID: A0A8B6LI76
• Organism: Variovorax sp. SRS16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1228080..1238934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5P2_RS38130	-	1228364..1228795 (+)	432	Protein_1178	NUDIX domain-containing protein	-
E5P2_RS05915 (G3W92_RS05915)	-	1228789..1228998 (-)	210	WP_162566221.1	DNA gyrase inhibitor YacG	-
E5P2_RS05920 (G3W92_RS05920)	zapD	1229072..1229827 (-)	756	WP_162566222.1	cell division protein ZapD	-
E5P2_RS05925 (G3W92_RS05925)	-	1229864..1230880 (-)	1017	WP_162566223.1	hypothetical protein	-
E5P2_RS05930 (G3W92_RS05930)	-	1230889..1232385 (-)	1497	WP_162566224.1	MBOAT family protein	-
E5P2_RS05935 (G3W92_RS05935)	coaE	1232467..1233066 (-)	600	WP_162566225.1	dephospho-CoA kinase	-
E5P2_RS05940 (G3W92_RS05940)	pilD	1233080..1233934 (-)	855	WP_162566226.1	A24 family peptidase	Machinery gene
E5P2_RS05945 (G3W92_RS05945)	pilC	1233934..1235157 (-)	1224	WP_162566227.1	type II secretion system F family protein	Machinery gene
E5P2_RS05950 (G3W92_RS05950)	pilB	1235178..1236911 (-)	1734	WP_162566228.1	type IV-A pilus assembly ATPase PilB	Machinery gene
E5P2_RS05960 (G3W92_RS05960)	-	1237145..1238137 (-)	993	WP_162566229.1	polyprenyl synthetase family protein	-
E5P2_RS05965 (G3W92_RS05965)	rplU	1238324..1238635 (+)	312	WP_162566230.1	50S ribosomal protein L21	-
E5P2_RS05970 (G3W92_RS05970)	rpmA	1238648..1238905 (+)	258	WP_162566231.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 284 a.a.
• Molecular weight: 30232.77 Da
• Isoelectric Point: 7.5465

>NTDB_id=1128203 E5P2_RS05940 WP_162566226.1 1233080..1233934(-) (pilD) [Variovorax sp. SRS16]
MLVSQGVDAALAGVFGLLIGSFLNVVIHRLPKMLERQWAAECASADGADAGAAAKDAEAFNLMVPHSRCPQCGHVIRWYE
NVPVLSYLFLRGRCSACKTPISLRYPVVELVTGALFAWCVWRMPGQFGSLAWCGFAAALLALALIDWDTTLLPDDITLPL
LWAGLLAAIAGFTGVTLVNAVWGAVAGYGSLWAIYWLFKLTTGKEGMGYGDFKLFAAFGVWFGWQALVPIILMASVIGVI
VGIALKLGKGLREGGYVPFGPFLAGAGFTAMIFGPDAILRVAGL

Nucleotide

• Length: 855 bp

>NTDB_id=1128203 E5P2_RS05940 WP_162566226.1 1233080..1233934(-) (pilD) [Variovorax sp. SRS16]
ATGCTGGTTTCGCAGGGGGTCGACGCCGCACTGGCCGGCGTGTTCGGGTTGTTGATCGGCAGTTTCCTCAACGTCGTGAT
CCATCGCCTGCCGAAGATGCTGGAGCGGCAATGGGCCGCCGAATGCGCGAGCGCCGACGGCGCGGACGCCGGGGCCGCGG
CGAAGGACGCCGAAGCTTTCAACCTGATGGTTCCGCACTCGCGCTGCCCACAGTGCGGCCATGTCATCCGCTGGTACGAG
AACGTCCCCGTCCTGAGCTACCTGTTCCTGCGCGGCCGATGCTCCGCGTGCAAGACCCCCATCAGCCTGCGCTATCCGGT
GGTCGAACTGGTGACCGGTGCGCTCTTCGCATGGTGTGTGTGGCGCATGCCCGGCCAGTTCGGCAGTCTGGCCTGGTGCG
GATTCGCTGCGGCGCTTCTTGCGCTGGCGCTGATCGACTGGGACACGACGCTGCTGCCGGACGACATCACGCTGCCGCTG
CTGTGGGCCGGCCTGCTGGCCGCCATCGCCGGCTTCACGGGCGTGACGCTCGTCAATGCCGTCTGGGGTGCAGTGGCGGG
CTATGGGTCGCTGTGGGCCATCTACTGGCTGTTCAAGCTGACCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGC
TGTTTGCCGCCTTCGGCGTATGGTTCGGATGGCAGGCCCTGGTGCCGATCATCCTCATGGCCTCGGTGATCGGCGTGATC
GTCGGCATCGCGCTCAAACTCGGCAAGGGCTTGCGCGAGGGCGGCTACGTGCCCTTCGGTCCATTCCTGGCGGGCGCGGG
CTTCACGGCCATGATCTTCGGTCCGGACGCGATCCTTCGCGTGGCAGGTCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-122)

3D structure

• AlphaFold DB: A0A8B6LI76

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.045	94.718	0.493
pilD	Vibrio campbellii strain DS40M4	49.438	94.014	0.465
pilD	Acinetobacter baumannii D1279779	45.018	95.423	0.43
pilD	Neisseria gonorrhoeae MS11	46.008	92.606	0.426
pilD	Acinetobacter nosocomialis M2	44.28	95.423	0.423