Detailed information NTG0112687

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSYRMG_RS04805
• Genome accession: NZ_CP005970
• Coordinates: 1190671..1191840 (-)
• Length: 389 a.a.
• NCBI ID: WP_003435515.1
• Uniprot ID: A0A8T8LR20
• Organism: Pseudomonas syringae UMAF0158
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1185671..1196840

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYRMG_RS04790 (PSYRMG_04955)	-	1186955..1188019 (+)	1065	WP_003364338.1	microcin C ABC transporter permease YejB	-
PSYRMG_RS04795 (PSYRMG_04960)	-	1188019..1189041 (+)	1023	WP_003435512.1	ABC transporter permease	-
PSYRMG_RS04800 (PSYRMG_04965)	-	1189043..1190620 (+)	1578	WP_046719327.1	ABC transporter ATP-binding protein	-
PSYRMG_RS04805 (PSYRMG_04970)	pilU	1190671..1191840 (-)	1170	WP_003435515.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSYRMG_RS04810 (PSYRMG_04975)	-	1191964..1192239 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
PSYRMG_RS04815 (PSYRMG_04980)	pgm	1192314..1193960 (-)	1647	WP_060707879.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSYRMG_RS04820 (PSYRMG_04985)	-	1194124..1196451 (-)	2328	WP_060707880.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42649.71 Da
• Isoelectric Point: 5.8676

>NTDB_id=112687 PSYRMG_RS04805 WP_003435515.1 1190671..1191840(-) (pilU) [Pseudomonas syringae UMAF0158]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=112687 PSYRMG_RS04805 WP_003435515.1 1190671..1191840(-) (pilU) [Pseudomonas syringae UMAF0158]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTACCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGAGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCCGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTACTGCTTGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACCGGTT
CGGGCAAGTCGACTTCGTTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGTCGTGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACTGAGCTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGCGAAGAAGAGGCCCTCAAGAACGCCGATTCACAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGTGAAGGCGGCGCCGGAACACTGACCACACCGCCACCGGCTCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGAGCCGGGTCCGCAAGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.331	99.486	0.56
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	50.829	93.059	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362