Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   FGK83_RS03330 Genome accession   NZ_LR590465
Coordinates   664771..666114 (-) Length   447 a.a.
NCBI ID   WP_138171617.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC8468     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 659771..671114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGK83_RS03300 (NCTC8468_00652) - 660566..660838 (-) 273 WP_005630954.1 HU family DNA-binding protein -
  FGK83_RS03305 (NCTC8468_00653) - 660977..661567 (-) 591 WP_138171613.1 YjaG family protein -
  FGK83_RS03310 (NCTC8468_00654) nudC 661603..662397 (-) 795 WP_138171614.1 NAD(+) diphosphatase -
  FGK83_RS03315 (NCTC8468_00655) nfuA 662465..663061 (-) 597 WP_114893180.1 Fe-S biogenesis protein NfuA -
  FGK83_RS03320 (NCTC8468_00656) - 663137..663823 (-) 687 WP_050847360.1 ComF family protein -
  FGK83_RS03325 (NCTC8468_00657) tnpA 664319..664774 (-) 456 WP_138171615.1 IS200/IS605 family transposase -
  FGK83_RS03330 (NCTC8468_00658) comE 664771..666114 (-) 1344 WP_138171617.1 type IV pilus secretin PilQ Machinery gene
  FGK83_RS03335 (NCTC8468_00659) comD 666124..666537 (-) 414 WP_138171618.1 pilus assembly protein PilP Machinery gene
  FGK83_RS03340 (NCTC8468_00660) comC 666534..667055 (-) 522 WP_138171620.1 competence protein ComC Machinery gene
  FGK83_RS03345 (NCTC8468_00661) comB 667052..667558 (-) 507 WP_114893185.1 competence protein B Machinery gene
  FGK83_RS03350 (NCTC8468_00662) comA 667559..668356 (-) 798 WP_114893186.1 pilus assembly protein PilM Machinery gene
  FGK83_RS03355 (NCTC8468_00663) - 668455..671055 (+) 2601 WP_138171622.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 49322.60 Da        Isoelectric Point: 8.1482

>NTDB_id=1126742 FGK83_RS03330 WP_138171617.1 664771..666114(-) (comE) [Haemophilus influenzae strain NCTC8468]
MKKYFLKCGCFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTVEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRSVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLAGNSFENISDNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVSYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKREDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGDTLDALKQKSAGKKLQ

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=1126742 FGK83_RS03330 WP_138171617.1 664771..666114(-) (comE) [Haemophilus influenzae strain NCTC8468]
ATGAAGAAATATTTTTTAAAGTGCGGTTGTTTTTTAGTATGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAACGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACAGTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATTGGAGATATATTGGAAAACAAGATCTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTACAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGGTTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAGGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTCAGCCACACGGTAAAACTCCATTTTGCTAAAGCCTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGATCTTTGCTTTCTTCTGCGGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTCTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGTCTTGCAGGCAATAGCTTTGAAAATATTTCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCGATAGCATTACAAGTCGCGAAAATTAATGGTCGATTGCTTGATTTAGAATT
GAGCGCTTTAGAGCGTGAAAATAATGTGGAAATTATCGCAAGTCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGTACAGAAATTCCTTATGTTGTGAGCAATACTCGTAATGATACACAATCTGTGGAATTTCGCGAGGCGGTG
CTTGGTTTGGAAGTCACACCACATATTTCTAAAGATAACAATATCTTGCTTGATTTATTAGTGAGCCAAAATTCTCCCGG
TTCTCGAGTTTCTTATGGACAAAACGAGGTGGTTTCTATTGATAAGCAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTACTTGGCGGTGTATTCCACGACACAATCACGAAAAGAGAAGATAAAGTGCCATTACTTGGCGATATA
CCCGTTATTAAACGATTATTCAGCAAAGAAAGCGAACGACATCAAAAACGTGAGTTAGTGATTTTCGTGACGCCGCATAT
TTTAAAAGCTGGAGATACGTTAGATGCTTTGAAACAAAAAAGTGCGGGGAAAAAGTTACAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(123-187)

Bacterial type II and III secretion system protein (Secretin)

(269-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

97.303

99.553

0.969
  comE Haemophilus influenzae 86-028NP

96.18

99.553

0.957
  comE Glaesserella parasuis strain SC1401

52.347

95.302

0.499
  pilQ Vibrio campbellii strain DS40M4

42.243

93.736

0.396
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.019

92.17

0.378
  pilQ Vibrio cholerae strain A1552

41.019

92.17

0.378

Multiple sequence alignment