Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   E0F34_RS10165 Genome accession   NZ_LR536843
Coordinates   1874102..1875118 (-) Length   338 a.a.
NCBI ID   WP_077141332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1869102..1880118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F34_RS12195 - 1869187..1869318 (-) 132 WP_000768904.1 hypothetical protein -
  E0F34_RS10125 (SAMEA104035599_01930) - 1869319..1870509 (-) 1191 WP_000167765.1 acetate kinase -
  E0F34_RS10130 (SAMEA104035599_01931) comYH 1870560..1871513 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  E0F34_RS10135 (SAMEA104035599_01932) - 1871574..1872161 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  E0F34_RS10140 (SAMEA104035599_01933) comGG/cglG 1872264..1872710 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F34_RS10145 (SAMEA104035599_01934) comGF/cglF 1872688..1873149 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F34_RS10150 (SAMEA104035599_01935) comGE/cglE 1873112..1873414 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F34_RS10155 (SAMEA104035599_01936) comGD/cglD 1873377..1873781 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F34_RS10160 (SAMEA104035599_01937) comGC/cglC 1873774..1874100 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F34_RS10165 (SAMEA104035599_01938) comGB/cglB 1874102..1875118 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F34_RS10170 (SAMEA104035599_01939) comGA/cglA/cilD 1875066..1876007 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F34_RS10175 (SAMEA104035599_01940) - 1876083..1876448 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F34_RS10180 (SAMEA104035599_01941) - 1876599..1877657 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F34_RS10185 (SAMEA104035599_01942) nagA 1877820..1878971 (-) 1152 WP_033705218.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38466.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1126591 E0F34_RS10165 WP_077141332.1 1874102..1875118(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1126591 E0F34_RS10165 WP_077141332.1 1874102..1875118(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment