Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   E0F34_RS10145 Genome accession   NZ_LR536843
Coordinates   1872688..1873149 (-) Length   153 a.a.
NCBI ID   WP_000250539.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1867688..1878149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F34_RS10115 (SAMEA104035599_01928) - 1868000..1868824 (-) 825 WP_000727909.1 membrane protein insertase YidC -
  E0F34_RS10120 (SAMEA104035599_01929) rnpA 1868799..1869170 (-) 372 WP_000739246.1 ribonuclease P protein component -
  E0F34_RS12195 - 1869187..1869318 (-) 132 WP_000768904.1 hypothetical protein -
  E0F34_RS10125 (SAMEA104035599_01930) - 1869319..1870509 (-) 1191 WP_000167765.1 acetate kinase -
  E0F34_RS10130 (SAMEA104035599_01931) comYH 1870560..1871513 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  E0F34_RS10135 (SAMEA104035599_01932) - 1871574..1872161 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  E0F34_RS10140 (SAMEA104035599_01933) comGG/cglG 1872264..1872710 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F34_RS10145 (SAMEA104035599_01934) comGF/cglF 1872688..1873149 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F34_RS10150 (SAMEA104035599_01935) comGE/cglE 1873112..1873414 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F34_RS10155 (SAMEA104035599_01936) comGD/cglD 1873377..1873781 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F34_RS10160 (SAMEA104035599_01937) comGC/cglC 1873774..1874100 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F34_RS10165 (SAMEA104035599_01938) comGB/cglB 1874102..1875118 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F34_RS10170 (SAMEA104035599_01939) comGA/cglA/cilD 1875066..1876007 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F34_RS10175 (SAMEA104035599_01940) - 1876083..1876448 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F34_RS10180 (SAMEA104035599_01941) - 1876599..1877657 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17851.34 Da        Isoelectric Point: 6.4832

>NTDB_id=1126587 E0F34_RS10145 WP_000250539.1 1872688..1873149(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1126587 E0F34_RS10145 WP_000250539.1 1872688..1873149(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae D39

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae R6

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae TIGR4

94.118

100

0.941
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915

Multiple sequence alignment