Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   E0F34_RS05620 Genome accession   NZ_LR536843
Coordinates   1037038..1037886 (-) Length   282 a.a.
NCBI ID   WP_000705303.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1032038..1042886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F34_RS05605 (SAMEA104035599_01062) - 1032322..1032681 (-) 360 WP_001237219.1 YbaN family protein -
  E0F34_RS05610 (SAMEA104035599_01063) topA 1032902..1034989 (-) 2088 WP_000179643.1 type I DNA topoisomerase -
  E0F34_RS05615 (SAMEA104035599_01064) - 1035156..1036199 (-) 1044 WP_001812710.1 TIGR00341 family protein -
  E0F34_RS05620 (SAMEA104035599_01065) dprA/cilB/dalA 1037038..1037886 (-) 849 WP_000705303.1 DNA-processing protein DprA Machinery gene
  E0F34_RS05625 (SAMEA104035599_01066) - 1037982..1038671 (-) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  E0F34_RS05630 (SAMEA104035599_01067) - 1038683..1039561 (-) 879 WP_000837609.1 DMT family transporter -
  E0F34_RS05635 (SAMEA104035599_01068) licA 1039551..1040420 (-) 870 WP_000411203.1 choline kinase LicA -
  E0F34_RS05640 (SAMEA104035599_01069) - 1040437..1041459 (-) 1023 WP_000609892.1 ribitol-5-phosphate dehydrogenase -
  E0F34_RS05645 (SAMEA104035599_01070) - 1041464..1042171 (-) 708 WP_000638501.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31076.75 Da        Isoelectric Point: 5.4047

>NTDB_id=1126553 E0F34_RS05620 WP_000705303.1 1037038..1037886(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1126553 E0F34_RS05620 WP_000705303.1 1037038..1037886(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAATCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTTTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGCTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.645

100

0.996
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

62.857

99.291

0.624
  dprA Lactococcus lactis subsp. cremoris KW2

55.357

99.291

0.55
  dprA Staphylococcus aureus N315

37.456

100

0.376

Multiple sequence alignment