Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OU5_RS21800 Genome accession   NZ_CP005960
Coordinates   4878744..4880444 (-) Length   566 a.a.
NCBI ID   WP_010467584.1    Uniprot ID   -
Organism   Pseudomonas mandelii JR-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4873744..4885444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU5_RS21765 (OU5_4359) - 4874397..4874864 (+) 468 WP_010467572.1 hypothetical protein -
  OU5_RS21770 (OU5_4360) - 4874861..4875550 (+) 690 WP_010467575.1 energy-coupling factor ABC transporter permease -
  OU5_RS21775 (OU5_4361) - 4875614..4875829 (+) 216 WP_010467577.1 hypothetical protein -
  OU5_RS21780 (OU5_4362) yacG 4875833..4876033 (-) 201 WP_010467579.1 DNA gyrase inhibitor YacG -
  OU5_RS21785 (OU5_4363) coaE 4876030..4876653 (-) 624 WP_042932554.1 dephospho-CoA kinase -
  OU5_RS21790 (OU5_4364) pilD 4876650..4877522 (-) 873 WP_010467582.1 prepilin peptidase Machinery gene
  OU5_RS21795 (OU5_4365) pilC 4877524..4878741 (-) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  OU5_RS21800 (OU5_4366) pilB 4878744..4880444 (-) 1701 WP_010467584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OU5_RS21805 (OU5_4367) pilA 4880670..4881077 (+) 408 WP_010467585.1 pilin Machinery gene
  OU5_RS21810 (OU5_4368) - 4881390..4881743 (+) 354 WP_010467586.1 BON domain-containing protein -
  OU5_RS21815 (OU5_4369) - 4882029..4882325 (-) 297 WP_010467587.1 DUF2845 domain-containing protein -
  OU5_RS21820 (OU5_4370) - 4882325..4885279 (-) 2955 WP_042932555.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62669.93 Da        Isoelectric Point: 5.8860

>NTDB_id=112650 OU5_RS21800 WP_010467584.1 4878744..4880444(-) (pilB) [Pseudomonas mandelii JR-1]
MNDIALSGLAKQLVMAELLTEQIAQQAYLQAQRSKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKEAQPRGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLGDAIEKFFDNQATGLEDMADVDLDG
LDIESVDDNKKDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPSQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIQGFNIATSVTLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=112650 OU5_RS21800 WP_010467584.1 4878744..4880444(-) (pilB) [Pseudomonas mandelii JR-1]
ATGAATGACATCGCCCTAAGCGGCCTGGCCAAGCAACTGGTCATGGCCGAACTGCTCACTGAGCAAATCGCGCAACAGGC
GTATCTGCAAGCCCAACGCAGCAAAATCTCTCTGGTCAGTTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTCGACCTCAATTGCCTGGACAAGGAAGCCCAGCCTCGAGGGTTG
GTCAGCGAGAAACTGATCCGTCAGCACCACGCCCTGCCCCTCTGGCGCCGTGGCAACAAACTGTTCGTGGGGGTTTCCGA
CCCGACCAATCATCAAGCCATCAATGACATCCAGTTCAGCACCGGTCTGAACACCGAAGCCATTCTGGTAGAGGACGATA
AGCTCGGCGATGCCATCGAGAAGTTCTTCGACAACCAAGCAACCGGCCTGGAAGACATGGCCGATGTTGACCTTGACGGG
CTGGACATCGAGTCGGTCGACGACAACAAAAAGGACGCCATCGCCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATGCTGCGAGAAGTCGCCAAGCCGCCGAGCCAACTGGCCAACCGCATTGCGGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCCAAGACCAA
GTCCATCGACTTCCGGGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAACAGAAAGCCTTGTACATGGCTGCACTGAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGGTTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCGCCCAGGCTCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTC
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGCTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGGC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGATCAGTGCAATGGCGGTTACAAGGGGCGAGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGACATTCAGA
TGCGCAAGGACGGCTTTAATGACTTGCGCACCTCGGGCTTGATCAAGGCCATGCAAGGCGTCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.117

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366

Multiple sequence alignment