Detailed information NTG0112648

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OU5_RS21790
• Genome accession: NZ_CP005960
• Coordinates: 4876650..4877522 (-)
• Length: 290 a.a.
• NCBI ID: WP_010467582.1
• Uniprot ID: A0A024EGH6
• Organism: Pseudomonas mandelii JR-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4871650..4882522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OU5_RS21750 (OU5_4356)	-	4872892..4873065 (-)	174	WP_008028730.1	DUF3094 domain-containing protein	-
OU5_RS21755 (OU5_4357)	-	4873117..4873644 (-)	528	WP_010467568.1	MOSC domain-containing protein	-
OU5_RS21760 (OU5_4358)	-	4873641..4874267 (-)	627	WP_007970534.1	DUF1780 domain-containing protein	-
OU5_RS21765 (OU5_4359)	-	4874397..4874864 (+)	468	WP_010467572.1	hypothetical protein	-
OU5_RS21770 (OU5_4360)	-	4874861..4875550 (+)	690	WP_010467575.1	energy-coupling factor ABC transporter permease	-
OU5_RS21775 (OU5_4361)	-	4875614..4875829 (+)	216	WP_010467577.1	hypothetical protein	-
OU5_RS21780 (OU5_4362)	yacG	4875833..4876033 (-)	201	WP_010467579.1	DNA gyrase inhibitor YacG	-
OU5_RS21785 (OU5_4363)	coaE	4876030..4876653 (-)	624	WP_042932554.1	dephospho-CoA kinase	-
OU5_RS21790 (OU5_4364)	pilD	4876650..4877522 (-)	873	WP_010467582.1	prepilin peptidase	Machinery gene
OU5_RS21795 (OU5_4365)	pilC	4877524..4878741 (-)	1218	WP_010467583.1	type II secretion system F family protein	Machinery gene
OU5_RS21800 (OU5_4366)	pilB	4878744..4880444 (-)	1701	WP_010467584.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OU5_RS21805 (OU5_4367)	pilA	4880670..4881077 (+)	408	WP_010467585.1	pilin	Machinery gene
OU5_RS21810 (OU5_4368)	-	4881390..4881743 (+)	354	WP_010467586.1	BON domain-containing protein	-
OU5_RS21815 (OU5_4369)	-	4882029..4882325 (-)	297	WP_010467587.1	DUF2845 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32141.49 Da
• Isoelectric Point: 8.2577

>NTDB_id=112648 OU5_RS21790 WP_010467582.1 4876650..4877522(-) (pilD) [Pseudomonas mandelii JR-1]
MPLNEVLILYPLVFVVIALAVGLLVGSFLNVVVWRLPKMLQREWRLQAHDVLGVPPEAPGTAYNLMLPHSHCPQCDHRIR
AWENIPLLSYLFLRGRCSNCAAPISKRYPLTELACGLLSGFIAWHFGFGWQACMVLVLTWGLLAMSLIDAEHQLLPDVLV
LPLLWLGLIVNSFGLFVSLHEALWGAVAGYMTLWSVFWLFKLLTGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
AIIGVILLRLRNAQTSTPIPFGPFLAIAGWIALLWGGQITGFYWQFVGLK

Nucleotide

• Length: 873 bp

>NTDB_id=112648 OU5_RS21790 WP_010467582.1 4876650..4877522(-) (pilD) [Pseudomonas mandelii JR-1]
ATGCCCTTGAACGAAGTCCTGATCCTCTACCCGCTGGTGTTCGTGGTCATCGCCTTGGCAGTCGGCCTGCTGGTAGGCAG
TTTCCTCAACGTGGTGGTCTGGCGCCTGCCGAAAATGCTCCAACGCGAATGGCGGCTGCAAGCCCATGACGTGTTGGGTG
TTCCGCCCGAGGCACCGGGCACCGCCTACAACTTGATGCTGCCTCATTCCCACTGCCCGCAGTGCGACCATCGAATCCGC
GCCTGGGAAAACATCCCGCTGCTCAGTTATCTGTTTTTACGGGGTCGATGCTCGAACTGCGCCGCGCCAATCAGCAAACG
TTATCCGCTGACTGAACTGGCCTGCGGTCTGCTCTCGGGGTTTATCGCCTGGCATTTCGGCTTCGGCTGGCAGGCCTGCA
TGGTGCTGGTGCTGACCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCTGAGCATCAACTGTTGCCGGATGTGCTGGTA
CTGCCATTGCTGTGGCTGGGGTTGATCGTCAACAGCTTCGGGCTGTTCGTGTCACTGCATGAGGCATTGTGGGGCGCAGT
GGCCGGTTATATGACCTTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTCACCGGCAAGGACGGCATCGGCCATGGCGATT
TCAAGTTACTGGCCCTGCTCGGGGCCTGGGGCGGCTGGCAGATATTGCCGTTGACCATTCTGCTGTCATCGCTCGTGGGG
GCCATCATCGGGGTGATTTTGCTGCGACTGCGCAACGCGCAAACCTCGACTCCGATCCCCTTCGGACCCTTTCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

3D structure

• AlphaFold DB: A0A024EGH6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.169	97.931	0.521
pilD	Vibrio campbellii strain DS40M4	52.837	97.241	0.514
pilD	Acinetobacter baumannii D1279779	49.635	94.483	0.469
pilD	Acinetobacter nosocomialis M2	49.635	94.483	0.469
pilD	Neisseria gonorrhoeae MS11	47.778	93.103	0.445