Detailed information NTG1126335

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: E0F40_RS04660
• Genome accession: NZ_LR216065
• Coordinates: 866118..866768 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387344.1
• Uniprot ID: A0A0T9GI09
• Organism: Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 861118..871768

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F40_RS04630	-	862098..862888 (+)	791	Protein_881	IS5 family transposase	-
E0F40_RS04640 (SAMEA3714487_00876)	-	863071..863571 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
E0F40_RS04645 (SAMEA3714487_00877)	-	863574..863900 (+)	327	Protein_883	TfoX/Sxy family protein	-
E0F40_RS04650 (SAMEA3714487_00879)	ald	864205..865304 (-)	1100	Protein_884	alanine dehydrogenase	-
E0F40_RS04655 (SAMEA3714487_00880)	-	865481..866050 (+)	570	WP_000443750.1	GNAT family N-acetyltransferase	-
E0F40_RS04660 (SAMEA3714487_00881)	comEA/celA/cilE	866118..866768 (+)	651	WP_000387344.1	helix-hairpin-helix domain-containing protein	Machinery gene
E0F40_RS04665 (SAMEA3714487_00882)	comEC/celB	866752..868992 (+)	2241	WP_000942394.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E0F40_RS04670	-	869171..869359 (+)	189	WP_001808514.1	hypothetical protein	-
E0F40_RS04675 (SAMEA3714487_00883)	-	869392..869979 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
E0F40_RS04680 (SAMEA3714487_00884)	-	869983..871167 (+)	1185	WP_000655960.1	ABC transporter permease	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23181.48 Da
• Isoelectric Point: 6.0746

>NTDB_id=1126335 E0F40_RS04660 WP_000387344.1 866118..866768(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1126335 E0F40_RS04660 WP_000387344.1 866118..866768(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGACT
CATCGACCGAAAAGGAAGTGAAGAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A0T9GI09

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	100	100	1
comEA/celA/cilE	Streptococcus pneumoniae Rx1	97.222	100	0.972
comEA/celA/cilE	Streptococcus pneumoniae D39	97.222	100	0.972
comEA/celA/cilE	Streptococcus pneumoniae R6	97.222	100	0.972
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis SK321	93.056	100	0.931
comEA	Lactococcus lactis subsp. cremoris KW2	41.778	100	0.435
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37