Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL265_RS04660 Genome accession   NZ_LR134515
Coordinates   1017742..1019136 (-) Length   464 a.a.
NCBI ID   WP_005601209.1    Uniprot ID   B0BPG4
Organism   Actinobacillus pleuropneumoniae strain NCTC10976     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1012742..1024136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL265_RS04630 (NCTC10976_00931) lptE 1013356..1013850 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  EL265_RS04635 (NCTC10976_00932) holA 1013959..1014984 (+) 1026 WP_005601200.1 DNA polymerase III subunit delta -
  EL265_RS04640 (NCTC10976_00933) yacG 1015061..1015249 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  EL265_RS04645 (NCTC10976_00934) coaE 1015231..1015869 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  EL265_RS04650 (NCTC10976_00935) - 1015891..1016529 (-) 639 WP_005604550.1 prepilin peptidase -
  EL265_RS04655 (NCTC10976_00936) pilC 1016556..1017758 (-) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  EL265_RS04660 (NCTC10976_00937) pilB 1017742..1019136 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  EL265_RS04665 (NCTC10976_00938) pilA 1019154..1019600 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL265_RS04670 (NCTC10976_00939) radA 1019782..1021161 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  EL265_RS04675 (NCTC10976_00940) - 1021205..1021438 (-) 234 WP_005597399.1 YgjV family protein -
  EL265_RS04680 (NCTC10976_00941) folA 1021565..1022053 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  EL265_RS04685 (NCTC10976_00942) proB 1022140..1023231 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  EL265_RS04690 (NCTC10976_00943) - 1023244..1024020 (+) 777 WP_005601215.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53022.90 Da        Isoelectric Point: 6.5042

>NTDB_id=1123687 EL265_RS04660 WP_005601209.1 1017742..1019136(-) (pilB) [Actinobacillus pleuropneumoniae strain NCTC10976]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1123687 EL265_RS04660 WP_005601209.1 1017742..1019136(-) (pilB) [Actinobacillus pleuropneumoniae strain NCTC10976]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATCTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

38.855

100

0.409
  pilB Vibrio cholerae strain A1552

39.004

100

0.405
  pilB Acinetobacter baylyi ADP1

38.32

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment