Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL259_RS03235 Genome accession   NZ_LR134510
Coordinates   703859..705307 (+) Length   482 a.a.
NCBI ID   WP_126598954.1    Uniprot ID   A0A448TT58
Organism   Actinobacillus delphinicola strain NCTC12871     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698859..710307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL259_RS03215 (NCTC12871_00655) dnaG 698896..700680 (+) 1785 WP_126598946.1 DNA primase -
  EL259_RS03220 (NCTC12871_00656) rpoD 700769..702673 (+) 1905 WP_126598948.1 RNA polymerase sigma factor RpoD -
  EL259_RS03225 (NCTC12871_00657) ampD 702739..703320 (-) 582 WP_126598950.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL259_RS03230 (NCTC12871_00658) pilA 703428..703850 (+) 423 WP_126598952.1 prepilin peptidase-dependent pilin Machinery gene
  EL259_RS03235 (NCTC12871_00659) pilB 703859..705307 (+) 1449 WP_126598954.1 GspE/PulE family protein Machinery gene
  EL259_RS03240 (NCTC12871_00660) - 705282..706493 (+) 1212 WP_172594213.1 type II secretion system F family protein -
  EL259_RS03245 (NCTC12871_00661) - 706497..707216 (+) 720 WP_126598958.1 A24 family peptidase -
  EL259_RS03250 (NCTC12871_00662) coaE 707247..707858 (+) 612 WP_126598960.1 dephospho-CoA kinase -
  EL259_RS03255 (NCTC12871_00663) - 708093..709649 (+) 1557 WP_126598961.1 glycerol-3-phosphate dehydrogenase/oxidase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 54444.47 Da        Isoelectric Point: 5.9141

>NTDB_id=1123562 EL259_RS03235 WP_126598954.1 703859..705307(+) (pilB) [Actinobacillus delphinicola strain NCTC12871]
MTINVSTPPCVFDVEGNQFQLSSERWQLNKSQYHILCRYLAIPLVEDNDTLWLGVESLENLQAVEIFSFLTAKTIEPVEL
CHEQLKALLQQLQTPQDGITLDTFYEPISPSPLSGGYSVEEEQKDGIILWLNQCFEDALKQRASDIHFNPQANNLMVYFR
IDGTLALYKKMPANSISRILSRLKLLAKLDISESRLPQDGHFQFKTPLSDYLEFRLSSLPTQFGEKIVLRLQHNTPIFTD
FSQLGMTEPQCTLFEWALRQPQGLILVTGPTGSGKSISLYSALARLNDGEKNILTAEDPIEVILPGITQSQINPAIQLSF
ARLLRTFLRQDPDIIMLGEIRDEESAKIALHAAQTGHLVLSTLHTNDAPSAIARLKQLGISQYELDHALLLIIAQRLVKR
ICPHCQKSSHKMKNCKCHNGYKGRIGIFQFLRPLPDEKEGAVYIDFPHLRESGLLKVQKGITDNDEIYRVLGNDYEESAT
FA

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=1123562 EL259_RS03235 WP_126598954.1 703859..705307(+) (pilB) [Actinobacillus delphinicola strain NCTC12871]
ATGACAATAAACGTTTCTACACCACCATGTGTCTTTGATGTTGAAGGTAATCAATTTCAACTGTCCAGCGAAAGGTGGCA
GTTAAATAAAAGCCAATACCACATTCTATGTCGATATCTCGCCATTCCACTCGTAGAAGATAATGATACGCTCTGGCTTG
GTGTAGAAAGTTTAGAAAATTTACAAGCCGTAGAGATATTTTCTTTTCTCACTGCGAAAACAATAGAACCCGTTGAATTA
TGCCATGAACAATTAAAAGCACTTTTACAACAACTCCAAACACCACAAGATGGTATAACACTTGATACCTTTTACGAACC
AATTTCACCATCACCTTTGTCTGGAGGTTATTCCGTTGAGGAAGAACAAAAAGATGGAATTATTCTATGGTTGAACCAAT
GCTTTGAAGATGCATTAAAACAACGTGCATCAGATATTCATTTTAATCCACAGGCAAATAATTTAATGGTTTACTTCCGT
ATTGATGGAACACTAGCACTTTATAAAAAAATGCCTGCTAACTCGATATCACGTATTCTTTCTCGCTTAAAACTACTTGC
GAAATTAGATATTAGTGAATCACGTTTACCACAAGACGGTCATTTTCAATTTAAAACGCCCTTATCTGATTACTTAGAAT
TTCGTCTTTCCTCACTACCAACTCAATTTGGAGAAAAAATTGTATTACGTTTACAACACAATACGCCTATTTTCACTGAT
TTTTCACAATTAGGAATGACAGAACCGCAATGTACTCTTTTTGAATGGGCCTTACGTCAACCCCAAGGACTTATTCTAGT
GACAGGACCGACTGGAAGTGGCAAAAGCATTTCACTTTACAGTGCATTAGCACGGCTTAATGATGGAGAAAAGAATATTC
TGACAGCAGAAGATCCCATTGAAGTCATCTTACCTGGCATTACACAAAGTCAAATAAATCCTGCTATCCAACTTTCTTTT
GCTCGCTTACTCCGTACTTTTCTACGGCAAGATCCCGATATTATTATGCTCGGCGAAATTCGTGATGAAGAAAGTGCTAA
GATCGCATTGCATGCTGCTCAAACAGGACATTTGGTTTTATCCACATTACATACAAACGATGCGCCTTCAGCTATCGCAC
GTTTAAAACAATTAGGCATTTCGCAATATGAATTAGATCACGCTCTTTTATTGATCATCGCTCAACGTTTAGTAAAAAGA
ATATGCCCACACTGCCAAAAATCATCACATAAAATGAAAAATTGTAAATGCCATAATGGATATAAAGGACGAATAGGTAT
TTTCCAATTTTTACGACCATTACCTGATGAGAAAGAAGGGGCTGTTTATATTGATTTTCCACATCTACGTGAGAGTGGTT
TACTTAAAGTCCAAAAGGGTATTACGGATAATGACGAAATTTATCGTGTATTAGGAAATGATTATGAAGAGTCAGCCACA
TTTGCATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-96)

Type II/IV secretion system protein (T2SSE)

(133-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A448TT58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

58.315

96.058

0.56
  pilB Haemophilus influenzae 86-028NP

58.315

96.058

0.56
  pilB Glaesserella parasuis strain SC1401

53.377

95.228

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

39.484

100

0.413
  pilB Vibrio campbellii strain DS40M4

39.034

100

0.402
  pilB Vibrio cholerae strain A1552

39.545

100

0.396
  pilB Legionella pneumophila strain ERS1305867

37.525

100

0.384
  pilB Acinetobacter baylyi ADP1

37.732

100

0.38
  pilB Acinetobacter baumannii D1279779

37.006

99.793

0.369

Multiple sequence alignment