Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I927_RS03610 Genome accession   NZ_CP004392
Coordinates   801043..801477 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida OH1905     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796043..806477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I927_RS03580 (I927_03590) - 796607..796876 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  I927_RS03585 (I927_03595) yacG 796876..797079 (-) 204 WP_046333438.1 DNA gyrase inhibitor YacG -
  I927_RS03590 (I927_03600) coaE 797090..797710 (-) 621 WP_046333439.1 dephospho-CoA kinase -
  I927_RS03595 (I927_03605) - 797725..798447 (-) 723 WP_046333440.1 prepilin peptidase -
  I927_RS03600 (I927_03610) - 798447..799658 (-) 1212 WP_046333441.1 type II secretion system F family protein -
  I927_RS03605 (I927_03615) pilB 799658..801043 (-) 1386 WP_046333442.1 GspE/PulE family protein Machinery gene
  I927_RS03610 (I927_03620) pilA 801043..801477 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  I927_RS03615 (I927_03625) ampD 801637..802203 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I927_RS03620 (I927_03630) rppH 802789..803385 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  I927_RS03625 (I927_03635) - 803388..804182 (+) 795 WP_046333443.1 sulfite exporter TauE/SafE family protein -
  I927_RS03630 (I927_03640) lgt 804192..804995 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  I927_RS03635 (I927_03645) - 805001..805852 (+) 852 WP_005751072.1 thymidylate synthase -
  I927_RS03640 (I927_03650) tadA 805867..806367 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=112346 I927_RS03610 WP_005741096.1 801043..801477(-) (pilA) [Pasteurella multocida OH1905]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=112346 I927_RS03610 WP_005741096.1 801043..801477(-) (pilA) [Pasteurella multocida OH1905]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment