Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL216_RS09105 Genome accession   NZ_LR134440
Coordinates   1939921..1941600 (+) Length   559 a.a.
NCBI ID   WP_085391168.1    Uniprot ID   A0A1X3CHA6
Organism   Neisseria animaloris strain NCTC12228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1934921..1946600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL216_RS09085 (NCTC12228_01833) - 1935626..1936609 (-) 984 WP_085391170.1 hypothetical protein -
  EL216_RS09090 (NCTC12228_01834) - 1937162..1938490 (+) 1329 WP_085391185.1 LysM peptidoglycan-binding domain-containing M23 family metallopeptidase -
  EL216_RS09095 (NCTC12228_01835) - 1938600..1939220 (-) 621 WP_085391169.1 isochorismatase family protein -
  EL216_RS09105 (NCTC12228_01837) pilF 1939921..1941600 (+) 1680 WP_085391168.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL216_RS09110 (NCTC12228_01838) pilG 1941639..1942877 (+) 1239 WP_085391167.1 type II secretion system F family protein Machinery gene
  EL216_RS09115 (NCTC12228_01839) pilD 1942882..1943745 (+) 864 WP_085391166.1 A24 family peptidase Machinery gene
  EL216_RS09120 (NCTC12228_01840) coaE 1943749..1944369 (+) 621 WP_085391165.1 dephospho-CoA kinase -
  EL216_RS09125 (NCTC12228_01841) zapD 1944366..1945133 (+) 768 WP_085391164.1 cell division protein ZapD -
  EL216_RS09130 (NCTC12228_01842) yacG 1945137..1945325 (+) 189 WP_085391163.1 DNA gyrase inhibitor YacG -
  EL216_RS09135 (NCTC12228_01843) - 1945380..1945823 (-) 444 WP_085391162.1 TIGR01244 family sulfur transferase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62200.84 Da        Isoelectric Point: 6.1401

>NTDB_id=1122869 EL216_RS09105 WP_085391168.1 1939921..1941600(+) (pilF) [Neisseria animaloris strain NCTC12228]
MSVGLLRVLVQGQVIDGNQAERYQDVLKANKPIIPMLFEEGVVTPKALGELLARVFSYPLLDLRHFSRNNILMDVLTEEQ
MLQNRCVPIFRRGRKVYLAVSDPTQIQNFQKIAFSSGVSIDLVVVCDDQLSTLLEWLGQRSTTILNEISKEQEAAAPTQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDTGSLNIDQLGFEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPSCKTPIERPPLAALKKAGFTDEDLAKDWTM
YRSVGCDSCRGKGYRGRAGVYEVMPITEEMQRVIMENGTEVDIMNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1122869 EL216_RS09105 WP_085391168.1 1939921..1941600(+) (pilF) [Neisseria animaloris strain NCTC12228]
ATGAGCGTCGGTTTATTAAGAGTATTGGTGCAAGGCCAAGTTATAGATGGAAATCAGGCAGAGCGGTATCAAGATGTATT
AAAAGCGAATAAGCCTATTATTCCGATGTTGTTTGAAGAAGGGGTAGTTACACCAAAAGCATTGGGTGAATTACTTGCAC
GAGTGTTCAGTTATCCGTTACTGGATCTCCGTCATTTTTCTCGTAATAACATTTTAATGGATGTTCTTACTGAAGAACAA
ATGCTGCAAAATCGTTGTGTGCCGATTTTTAGACGGGGTAGAAAAGTCTATTTGGCAGTATCTGATCCCACCCAAATTCA
AAATTTTCAGAAAATTGCTTTTTCATCAGGTGTTTCAATTGACTTGGTTGTGGTATGTGATGATCAGTTGAGTACATTAT
TGGAATGGTTAGGGCAGCGTTCCACCACTATTTTGAATGAAATAAGTAAGGAACAAGAAGCTGCCGCTCCTACACAAGCT
CTTTATATTGATAATGAAGAAGCTGAAGATGGGCCAATTCCGCGCTTTATTCATAAAACTCTGTCTGACGCGCTTAATGC
AGGAGCTTCAGATATCCACTTTGAATTTTATGAACAGATGGCACGGGTGCGTTTCCGTGTCGATGGGCAATTGCGTGAAG
TTGTCCAGCCTCCAGTAGCAGTGAGAGGGCAATTGGCTTCGCGCATCAAAGTGATGGCAAGGTTGGATATTTCGGAAAAG
CGTGTGCCCCAAGATGGACGTATTCAAATTGCCTTTCATAAGCATGGTCGTCCGATTGATTTTCGTGTGAGTACGTTACC
GACCTTGTTCGGTGAAAAAGTAGTAATGCGTATTTTGAATTCGGATACAGGGTCTTTAAATATTGATCAACTTGGTTTTG
AACCTTTCCAAAAGGAAATGCTGTTGGAGGCTATTAACCGCCCTTATGGAATGGTGTTGGTTACCGGCCCGACAGGCTCG
GGTAAAACGGTATCTCTTTATACTTGTTTGAATATTTTAAATACAGAGAATGTTAACATTGCTACTGCAGAAGACCCTGC
AGAAATTAACTTGCCGGGTATTAATCAGGTTAATGTGAATGACAAACAAGGATTAACATTTGCTGCAGCTTTGAAAGCAT
TCCTACGTCAAGATCCGGATATTATTATGGTTGGTGAGATCCGAGACTTAGAAACAGCTGATATTGCTATTAAGGCAGCG
CAAACAGGACATATGGTATTTTCTACACTGCATACGAATAATGCACCAGCCACTTTGTCACGGATGTTAAATATGGGGGT
GGCACCATTCAATATTGCCAGTTCGGTAAGTTTGATTATGGCTCAACGGTTATTGCGTCGATTATGTCCCTCTTGTAAAA
CTCCCATTGAACGTCCTCCTTTGGCAGCATTAAAGAAAGCGGGTTTTACTGATGAAGATTTAGCCAAAGACTGGACAATG
TACCGTTCGGTTGGTTGCGATAGCTGTCGAGGTAAGGGTTATAGAGGCCGTGCAGGTGTCTATGAAGTGATGCCTATTAC
TGAAGAAATGCAACGGGTAATCATGGAAAATGGCACTGAAGTTGATATTATGAATATGGCCTATAAAGAAGGTATGGTGG
ATCTGCGTCGTGCAGGTTTACTAAAAGTAATGCAAGGGATTACTTCATTAGAAGAAGTACTCGCACATACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3CHA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.175

100

0.782
  pilB Acinetobacter baylyi ADP1

51.619

99.463

0.513
  pilB Acinetobacter baumannii D1279779

51.079

99.463

0.508
  pilB Legionella pneumophila strain ERS1305867

47.143

100

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio cholerae strain A1552

45.035

100

0.454
  pilB Vibrio campbellii strain DS40M4

43.085

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.815

93.56

0.363

Multiple sequence alignment