Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL200_RS21175 Genome accession   NZ_LR134301
Coordinates   4556089..4557816 (+) Length   575 a.a.
NCBI ID   WP_057499447.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain NCTC13014     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4551089..4562816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL200_RS21160 (NCTC13014_04216) - 4551476..4551913 (+) 438 WP_057499450.1 pilin -
  EL200_RS21165 (NCTC13014_04217) - 4552012..4552887 (+) 876 WP_160320739.1 sterol desaturase family protein -
  EL200_RS21170 (NCTC13014_04218) - 4553019..4556075 (+) 3057 WP_057499448.1 fused MFS/spermidine synthase -
  EL200_RS21175 (NCTC13014_04219) pilB 4556089..4557816 (+) 1728 WP_057499447.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL200_RS21180 (NCTC13014_04220) pilR 4557922..4559295 (-) 1374 WP_049447478.1 sigma-54 dependent transcriptional regulator Regulator
  EL200_RS21185 (NCTC13014_04221) - 4559356..4560969 (-) 1614 WP_049447477.1 ATP-binding protein -
  EL200_RS21190 (NCTC13014_04222) sucC 4561375..4562544 (+) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62306.32 Da        Isoelectric Point: 5.8743

>NTDB_id=1120739 EL200_RS21175 WP_057499447.1 4556089..4557816(+) (pilB) [Stenotrophomonas maltophilia strain NCTC13014]
MSVVSPPNLVGITGLARRLVQDGALDEATARDAMARAAAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVATSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEHA
LLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1120739 EL200_RS21175 WP_057499447.1 4556089..4557816(+) (pilB) [Stenotrophomonas maltophilia strain NCTC13014]
ATGAGCGTTGTTTCCCCCCCCAACCTCGTCGGCATCACCGGCCTGGCCCGCCGCCTGGTCCAGGACGGCGCCCTCGACGA
GGCTACCGCCCGCGACGCGATGGCCAGGGCCGCCGCCGCCCGCCAGCCGCTGCCCACCTGGTTCGCCCAGAACAAGCTGG
TCAGCGCCTCGCAGCTCGCCGCCGCCAACGCTGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAATGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTCGCTACCAGCAATCCGACCCATGCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAACCGATCCTGG
TTGATGAAGACCAGATCCGCCGGACGCTGGAGCAGTGGCACGCCAGCCACGACACCATCGGCGACGCGCTCGGTGGCGAT
GACGAGGGTATGGCCAACCTCGATGTCGGGTTGGGTGACGAGGACAATGCCGGTGGCGACTCCGGCATCGACGCCAAGGG
TGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCAAGGGCGCATCGGACATCCACTTCG
AACCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCAGTGAAGTTG
AACCAACGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGTCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAGGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTGTACACGGC
GCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
GCTGCATACCAATGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCGCAGCGCCTGGCGCGCCGGTTGTGCAACAACTGCAAGCGCCCGGCGAATCTGCCGGAGCATGCA
TTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGACGCAGGCATTGAGCTGTACGAGGCGGTGGGGTGCGATGAGTGCAC
CGAGGGCTACAAGGGCCGTACCGGTCTTTACCAGGTGATGCCGATGACCGATGAGATCGCTACGATCGTGCTGGCCGGTG
GCAATGCACTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCG
GCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.143

98.609

0.563
  pilB Acinetobacter baylyi ADP1

55.633

100

0.558
  pilB Legionella pneumophila strain ERS1305867

52.189

99.304

0.518
  pilF Neisseria gonorrhoeae MS11

49.383

98.609

0.487
  pilB Vibrio cholerae strain A1552

49.293

98.435

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.06

99.304

0.457
  pilB Vibrio campbellii strain DS40M4

44.815

98.957

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

36.334

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

93.043

0.363

Multiple sequence alignment