Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   EL096_RS03620 Genome accession   NZ_LR134294
Coordinates   699932..700780 (-) Length   282 a.a.
NCBI ID   WP_000705304.1    Uniprot ID   A0A0E7P1X9
Organism   Streptococcus pneumoniae strain NCTC12977     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 694932..705780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL096_RS03605 (NCTC12977_00704) - 695286..695645 (-) 360 WP_001237219.1 YbaN family protein -
  EL096_RS03610 (NCTC12977_00705) topA 695751..697838 (-) 2088 WP_000179644.1 type I DNA topoisomerase -
  EL096_RS03615 (NCTC12977_00706) - 698005..699048 (-) 1044 WP_001842104.1 TIGR00341 family protein -
  EL096_RS03620 (NCTC12977_00707) dprA/cilB/dalA 699932..700780 (-) 849 WP_000705304.1 DNA-processing protein DprA Machinery gene
  EL096_RS03625 (NCTC12977_00708) - 700876..701565 (-) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  EL096_RS03630 (NCTC12977_00709) - 701577..702455 (-) 879 WP_000837618.1 DMT family transporter -
  EL096_RS03635 (NCTC12977_00710) licA 702445..703314 (-) 870 WP_000411215.1 choline kinase LicA -
  EL096_RS03640 (NCTC12977_00711) - 703331..704353 (-) 1023 WP_000609885.1 ribitol-5-phosphate dehydrogenase -
  EL096_RS03645 (NCTC12977_00712) - 704358..705065 (-) 708 WP_000638502.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31076.75 Da        Isoelectric Point: 5.4047

>NTDB_id=1120539 EL096_RS03620 WP_000705304.1 699932..700780(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain NCTC12977]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1120539 EL096_RS03620 WP_000705304.1 699932..700780(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain NCTC12977]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E7P1X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.645

100

0.996
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.357

99.291

0.55
  dprA Staphylococcus aureus N315

37.456

100

0.376

Multiple sequence alignment