Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOA_RS07440 Genome accession   NZ_CP004006
Coordinates   1516430..1518151 (+) Length   573 a.a.
NCBI ID   WP_025385785.1    Uniprot ID   -
Organism   Legionella oakridgensis ATCC 33761 = DSM 21215     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1511430..1523151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOA_RS07415 (Loa_01657) - 1513012..1513587 (+) 576 WP_035894210.1 hypothetical protein -
  LOA_RS07420 (Loa_01658) - 1513694..1514395 (+) 702 WP_025385781.1 L,D-transpeptidase family protein -
  LOA_RS07425 (Loa_01659) - 1514407..1514967 (-) 561 WP_025385782.1 hypoxanthine-guanine phosphoribosyltransferase -
  LOA_RS07430 (Loa_01660) - 1514994..1515320 (-) 327 WP_025385783.1 DUF1820 family protein -
  LOA_RS07435 (Loa_01661) - 1515419..1516180 (-) 762 WP_025385784.1 methyltransferase domain-containing protein -
  LOA_RS07440 (Loa_01662) pilB 1516430..1518151 (+) 1722 WP_025385785.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOA_RS07445 (Loa_01663) pilC 1518156..1519373 (+) 1218 WP_025385786.1 type II secretion system F family protein Machinery gene
  LOA_RS07450 (Loa_01664) - 1519377..1520264 (+) 888 WP_025385787.1 prepilin peptidase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63370.60 Da        Isoelectric Point: 5.6942

>NTDB_id=112002 LOA_RS07440 WP_025385785.1 1516430..1518151(+) (pilB) [Legionella oakridgensis ATCC 33761 = DSM 21215]
MSLMPEEHLQGIAQLLVQEHLLDKDNALKYQQLATRNKQSLQQYLVDSNIISAKQIALSVAQNFGVPVLDLDCIDIDSIP
TTLVSDKLIQRHGLIPLFSRGKQLYIAIDDPSKHASLKEIQFHTGLHAMPLIVETDKLNELIDKLIHEKESQGLASYFDE
TKDIDGLEFSTDEEDNEADTSGLSSEDAPIVKFVNRIIVEAIKKSASDIHFEPYERSYRIRYRQDGILNEITTPPISLAA
RISARIKVMSNLDISERRIPQDGRFKMKLSPTRSVDFRVSTCPTVAGEKVVMRILDPGSTKLGVESLGFNPKQKEHFLQA
IQRPQGMILVTGPTGSGKTVTLYSALNTLNTTENNISTAEDPVEIKVPGINQVNINTKAGLTFSSALRAFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLVNMGIPSFNIASSVSLIIAQRLVRRLCDQCKVIRDDFTIASL
IELGFSEVEANTLKAYKANGCSQCTNGYRGRIGLFEVLPMTKNLGRLIMSGGNSLDILKQAQEEGMLTVYQSGLEKIKEG
ITTIEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=112002 LOA_RS07440 WP_025385785.1 1516430..1518151(+) (pilB) [Legionella oakridgensis ATCC 33761 = DSM 21215]
ATGTCCCTTATGCCAGAAGAGCATTTACAAGGTATTGCTCAGCTTTTAGTTCAAGAACATTTACTTGATAAAGATAATGC
TTTGAAATATCAGCAACTTGCAACACGTAATAAGCAAAGTCTTCAACAATACTTGGTGGATAGTAACATTATTTCAGCCA
AACAGATTGCTTTGTCAGTAGCCCAAAACTTTGGTGTCCCGGTTCTTGATCTGGATTGTATCGATATTGATTCAATACCT
ACCACGTTAGTCAGCGATAAATTAATTCAACGTCATGGTCTGATACCCTTATTCAGTCGCGGAAAACAATTATACATAGC
CATAGATGATCCAAGTAAACATGCCTCATTGAAAGAAATTCAGTTTCATACCGGTTTACATGCCATGCCATTGATTGTTG
AAACGGATAAATTAAATGAATTGATTGATAAACTCATTCACGAAAAAGAAAGTCAGGGCTTAGCCTCTTATTTTGATGAA
ACCAAAGATATTGACGGCTTGGAATTCAGCACGGATGAAGAAGATAATGAAGCAGATACGTCCGGTTTATCAAGTGAAGA
TGCGCCCATCGTAAAATTTGTGAATAGAATCATCGTTGAAGCCATAAAAAAAAGCGCTTCTGATATTCATTTTGAGCCTT
ATGAACGCAGCTATCGTATCCGTTACCGGCAAGATGGGATCCTCAATGAAATCACCACACCGCCAATCAGCCTGGCGGCT
CGGATTTCTGCCCGTATTAAGGTCATGTCAAATCTGGATATTTCTGAACGTCGTATTCCGCAAGATGGCCGCTTTAAGAT
GAAATTGTCACCAACCCGCTCTGTTGACTTTCGAGTGAGCACTTGTCCAACAGTGGCTGGCGAAAAAGTGGTGATGAGAA
TCCTCGACCCTGGCTCAACCAAGCTTGGAGTTGAATCCCTGGGATTTAACCCAAAACAAAAAGAACACTTTTTACAAGCC
ATTCAGCGGCCTCAGGGAATGATTCTCGTTACCGGCCCTACAGGAAGCGGTAAAACTGTAACTCTCTATTCCGCCTTAAA
CACACTCAATACTACTGAAAATAATATTTCAACGGCGGAAGATCCAGTGGAAATTAAGGTCCCAGGCATTAATCAAGTGA
ATATCAATACAAAAGCCGGCCTTACTTTTTCCAGCGCTTTACGTGCTTTTTTACGTCAAGATCCGGATATTATCATGGTT
GGTGAAATACGGGATTTGGAAACCGCAGAAATAGCCATTAAAGCCGCCCAAACCGGTCACTTGGTTTTATCCACTTTGCA
TACCAACAGTGCTGCTGAAACATTGACTCGTCTGGTCAATATGGGCATTCCTTCTTTTAATATTGCAAGCTCCGTAAGCT
TGATTATTGCTCAACGGTTGGTGAGGCGTTTGTGCGATCAATGCAAGGTTATACGTGATGATTTCACCATTGCCAGTTTA
ATTGAACTTGGTTTTTCAGAAGTTGAAGCAAATACTCTGAAAGCCTACAAAGCTAATGGTTGCAGCCAATGCACTAATGG
TTATCGCGGGCGTATTGGCTTATTTGAAGTCTTGCCCATGACTAAAAATCTTGGCCGCTTAATTATGTCTGGCGGAAATT
CTCTCGATATTCTCAAGCAAGCCCAAGAAGAAGGCATGCTCACCGTCTATCAATCCGGGCTTGAAAAAATTAAAGAAGGT
ATTACAACGATTGAAGAAGTCAATCGGGTAACTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

73.868

100

0.74
  pilB Acinetobacter baumannii D1279779

50.709

98.429

0.499
  pilB Acinetobacter baylyi ADP1

49.824

99.127

0.494
  pilB Vibrio cholerae strain A1552

47.778

94.241

0.45
  pilF Neisseria gonorrhoeae MS11

45.583

98.778

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

47.048

94.59

0.445
  pilB Vibrio campbellii strain DS40M4

47.29

93.368

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.364

95.986

0.387
  pilF Thermus thermophilus HB27

38.489

99.302

0.382

Multiple sequence alignment