Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ELZ66_RS04795 Genome accession   NZ_LR133933
Coordinates   1033581..1035677 (+) Length   698 a.a.
NCBI ID   WP_010946666.1    Uniprot ID   Q5ZX02
Organism   Legionella pneumophila strain NCTC12180     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1028581..1040677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ66_RS04770 (NCTC12180_00946) ravI 1029337..1030347 (-) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -
  ELZ66_RS04775 (NCTC12180_00947) pilM 1030764..1031828 (+) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ELZ66_RS04780 (NCTC12180_00948) pilN 1031832..1032380 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ELZ66_RS04785 (NCTC12180_00949) pilO 1032394..1032993 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ELZ66_RS04790 (NCTC12180_00950) pilP 1032990..1033577 (+) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ELZ66_RS04795 (NCTC12180_00951) pilQ 1033581..1035677 (+) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ELZ66_RS04800 (NCTC12180_00952) aroK 1036129..1036650 (+) 522 WP_027219629.1 shikimate kinase AroK -
  ELZ66_RS04805 (NCTC12180_00953) aroB 1036637..1037746 (+) 1110 WP_010946668.1 3-dehydroquinate synthase -
  ELZ66_RS04810 (NCTC12180_00954) - 1037743..1039194 (+) 1452 WP_010946669.1 SPOR domain-containing protein -
  ELZ66_RS04815 (NCTC12180_00955) - 1039303..1039734 (+) 432 WP_010946670.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77487.79 Da        Isoelectric Point: 9.5345

>NTDB_id=1117694 ELZ66_RS04795 WP_010946666.1 1033581..1035677(+) (pilQ) [Legionella pneumophila strain NCTC12180]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSIYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=1117694 ELZ66_RS04795 WP_010946666.1 1033581..1035677(+) (pilQ) [Legionella pneumophila strain NCTC12180]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTGCGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCTGCAA
GCTTTATTACTCAAAAGCCGCCCCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTATCCTATTCTGGCAGCACGGCGGGTTCCATTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCACGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGTGGTAGAGTCTTAATTGACGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAGAAGGTTCGCATGACCATTCTCAGCAAAGGGGATTATGGACATTTTGTGTAT
CAGGTCAATAAACAATTTATGGTTGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTAAAGAAACAGGT
ATTTACCGGTAAACGTATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTACTTCAATTGCTGGCAGATTTTACTG
GAATCAATATGGTTGTCAGCGACAAAGTACAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAAGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACCCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCAGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATACCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCGGTATTGAGTTTGAAAGTTACCCCACAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAGGAATTACTGATTTTTATTACTCCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAAA
AGGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZX02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

100

100

1
  pilQ Legionella pneumophila strain ERS1305867

99.57

100

0.996
  pilQ Pseudomonas aeruginosa PAK

37.73

100

0.381
  comQ Acinetobacter baylyi ADP1

38.428

98.424

0.378
  pilQ Acinetobacter baumannii strain A118

35.095

100

0.371
  pilQ Acinetobacter baumannii D1279779

35.095

100

0.371

Multiple sequence alignment