Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF   Type   Machinery gene
Locus tag   E0E15_RS08235 Genome accession   NZ_LR027870
Coordinates   1631379..1631876 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain BPH2019 isolate BPH2019     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1626379..1636876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E15_RS08210 gcvPB 1626542..1628014 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  E0E15_RS08215 gcvPA 1628007..1629353 (-) 1347 WP_000019684.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E0E15_RS08220 gcvT 1629373..1630464 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  E0E15_RS08225 - 1630623..1631147 (-) 525 WP_116100530.1 shikimate kinase -
  E0E15_RS08230 - 1631137..1631282 (-) 146 Protein_1544 hypothetical protein -
  E0E15_RS08235 comGF 1631379..1631876 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0E15_RS08240 comGE 1631794..1632093 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E0E15_RS08245 comGD 1632080..1632526 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0E15_RS08250 comGC 1632504..1632815 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E0E15_RS08260 - 1632984..1634156 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  E0E15_RS08265 comGB 1634140..1635231 (-) 1092 WP_130827171.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0E15_RS08270 comGA 1635203..1636177 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E0E15_RS08275 - 1636229..1636852 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1115829 E0E15_RS08235 WP_001803317.1 1631379..1631876(-) (comGF) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1115829 E0E15_RS08235 WP_001803317.1 1631379..1631876(-) (comGF) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994

  comGF Staphylococcus aureus N315

95.758

100

0.958


Multiple sequence alignment