Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E0E15_RS08270 Genome accession   NZ_LR027870
Coordinates   1635203..1636177 (-) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus strain BPH2019 isolate BPH2019     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1630203..1641177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E15_RS08225 - 1630623..1631147 (-) 525 WP_116100530.1 shikimate kinase -
  E0E15_RS08230 - 1631137..1631282 (-) 146 Protein_1544 hypothetical protein -
  E0E15_RS08235 comGF 1631379..1631876 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0E15_RS08240 comGE 1631794..1632093 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E0E15_RS08245 comGD 1632080..1632526 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0E15_RS08250 comGC 1632504..1632815 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E0E15_RS08260 - 1632984..1634156 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  E0E15_RS08265 comGB 1634140..1635231 (-) 1092 WP_130827171.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0E15_RS08270 comGA 1635203..1636177 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E0E15_RS08275 - 1636229..1636852 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E0E15_RS08280 - 1636849..1637178 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E0E15_RS08285 - 1637178..1638164 (-) 987 WP_000161314.1 ROK family glucokinase -
  E0E15_RS08290 - 1638161..1638364 (-) 204 WP_000087561.1 YqgQ family protein -
  E0E15_RS08295 - 1638345..1639808 (-) 1464 WP_001017867.1 rhomboid family protein -
  E0E15_RS08300 - 1639820..1640359 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  E0E15_RS08305 rpmG 1640568..1640717 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=1115834 E0E15_RS08270 WP_000697228.1 1635203..1636177(-) (comGA) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1115834 E0E15_RS08270 WP_000697228.1 1635203..1636177(-) (comGA) [Staphylococcus aureus strain BPH2019 isolate BPH2019]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991

Multiple sequence alignment