Detailed information NTG1115435

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAMMD1_RS11495
• Genome accession: NZ_LN999829
• Coordinates: 2474812..2475426 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain B25
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2469812..2480426

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMMD1_RS11475 (BAMMD1_2313)	rpsT	2470112..2470378 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAMMD1_RS11480 (BAMMD1_2314)	holA	2470395..2471436 (-)	1042	Protein_2299	DNA polymerase III subunit delta	-
BAMMD1_RS19235	-	2471476..2471630 (-)	155	Protein_2300	hypothetical protein	-
BAMMD1_RS18855 (BAMMD1_2315)	-	2471671..2471805 (+)	135	WP_003152870.1	YqzM family protein	-
BAMMD1_RS11485 (BAMMD1_2316)	comEC	2471824..2474175 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAMMD1_RS11490 (BAMMD1_2317)	-	2474176..2474745 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAMMD1_RS11495 (BAMMD1_2318)	comEA	2474812..2475426 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAMMD1_RS11500 (BAMMD1_2319)	comER	2475485..2476306 (+)	822	WP_014305449.1	late competence protein ComER	-
BAMMD1_RS11505 (BAMMD1_2320)	-	2476375..2477112 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
BAMMD1_RS11510 (BAMMD1_2321)	rsfS	2477109..2477465 (-)	357	WP_014305451.1	ribosome silencing factor	-
BAMMD1_RS11515 (BAMMD1_2322)	yqeK	2477462..2478043 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAMMD1_RS11520 (BAMMD1_2323)	-	2478033..2478602 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
BAMMD1_RS11525 (BAMMD1_2324)	yhbY	2478613..2478903 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAMMD1_RS11530 (BAMMD1_2325)	aroE	2478897..2479739 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1115435 BAMMD1_RS11495 WP_003152867.1 2474812..2475426(-) (comEA) [Bacillus velezensis strain B25]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1115435 BAMMD1_RS11495 WP_003152867.1 2474812..2475426(-) (comEA) [Bacillus velezensis strain B25]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363