Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KKKWG1_RS06540 Genome accession   NZ_LN869922
Coordinates   1279779..1280963 (-) Length   394 a.a.
NCBI ID   WP_019389075.1    Uniprot ID   -
Organism   Kingella kingae strain KWG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1274779..1285963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKKWG1_RS06500 (KKKWG1_1322) tatC 1275686..1276450 (-) 765 WP_003786159.1 twin-arginine translocase subunit TatC -
  KKKWG1_RS06505 (KKKWG1_1323) tatB 1276447..1276953 (-) 507 WP_019389080.1 Sec-independent protein translocase protein TatB -
  KKKWG1_RS06510 (KKKWG1_1324) tatA 1276955..1277158 (-) 204 WP_003786162.1 Sec-independent protein translocase subunit TatA -
  KKKWG1_RS06515 (KKKWG1_1325) - 1277210..1277530 (-) 321 WP_019389079.1 histidine triad nucleotide-binding protein -
  KKKWG1_RS06520 (KKKWG1_1326) - 1277602..1277931 (-) 330 WP_019389078.1 phosphoribosyl-ATP diphosphatase -
  KKKWG1_RS06525 (KKKWG1_1327) hisI 1277959..1278354 (-) 396 WP_003790152.1 phosphoribosyl-AMP cyclohydrolase -
  KKKWG1_RS06530 (KKKWG1_1328) - 1278364..1278894 (-) 531 WP_019389077.1 CreA family protein -
  KKKWG1_RS06535 (KKKWG1_1329) - 1278909..1279661 (-) 753 WP_019389076.1 sulfite exporter TauE/SafE family protein -
  KKKWG1_RS06540 (KKKWG1_1330) pilU 1279779..1280963 (-) 1185 WP_019389075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KKKWG1_RS06545 (KKKWG1_1331) - 1281145..1281627 (+) 483 WP_032133631.1 Lrp/AsnC family transcriptional regulator -
  KKKWG1_RS06550 (KKKWG1_1332) - 1281733..1283073 (+) 1341 WP_019389073.1 DUF3482 domain-containing protein -
  KKKWG1_RS06555 (KKKWG1_1333) - 1283236..1284081 (+) 846 WP_003786171.1 PHP domain-containing protein -
  KKKWG1_RS06560 (KKKWG1_1334) - 1284204..1285655 (+) 1452 WP_019389072.1 sodium:proton antiporter -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43728.24 Da        Isoelectric Point: 6.0102

>NTDB_id=1114882 KKKWG1_RS06540 WP_019389075.1 1279779..1280963(-) (pilU) [Kingella kingae strain KWG1]
MQVIIPPINEEMRKFIHGLLTHMVNNKGSDLFITAGYPPAMKLDGKLTKITDKPLTADHAMQIARSIMDDKQAEEFLTTN
ECNFAISLPGVSRFRINAMVQRGAAALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMAT
LHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKKQGKGRVAAVEILLNSPLISELVLHGDIHAVKEIMA
KSRDIGMQTFDQSLFDLYEADLISYDDALRNADSVNDLRLQIQLNSKKNATAGSGGVLDGLSLTDYAPPEAEEK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=1114882 KKKWG1_RS06540 WP_019389075.1 1279779..1280963(-) (pilU) [Kingella kingae strain KWG1]
ATGCAAGTTATCATTCCACCCATCAACGAAGAAATGCGTAAATTTATCCATGGCTTGCTCACGCACATGGTCAATAACAA
AGGCTCGGATTTGTTCATCACGGCTGGCTATCCACCTGCGATGAAATTGGACGGCAAATTAACCAAAATCACCGACAAGC
CTTTAACCGCCGACCACGCCATGCAAATTGCGCGTTCGATTATGGACGACAAGCAAGCCGAAGAATTTTTAACCACCAAC
GAATGCAATTTTGCGATTAGCTTGCCAGGTGTATCGCGTTTTCGAATCAACGCCATGGTGCAACGTGGTGCAGCAGCTTT
GGTTTGTCGTGTGATTACCAGCGACATTCCCAAATTCGACAACATGAACTTACCGCCTGTGTTAAAACAAGTCGTGATGG
AAAAACGTGGTTTGGTGATTTTTGTCGGCGGTACAGGTTCGGGTAAATCCACATCGTTGGCGGCGATGATTGATTACCGC
AACGAAAATAGCTACGGTCATATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACAAAAACTGCATCATCAC
GCAACGCGAAGTGGGTGTGGACACCGAAAACTGGTTCGCCGCGCTCAAAAACACGCTGCGCCAAGCACCCGATGTGATTT
TGATTGGTGAGATTCGTGACCGCGAAACGATGGACTACGCACTTGCCTTTGCCGAAACAGGACACTTGTGTATGGCAACG
CTGCACGCGAATAACTCCAACCAGGCCCTTGACCGCATTATTAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGAA
CGACTTGTCGCTTAACTTAAAAGGCTTTATTTCGCAACGCCTTGTCCCCAAAAAACAAGGCAAAGGACGTGTGGCTGCGG
TAGAAATCCTGCTCAATTCACCGTTGATTTCCGAATTGGTGTTGCATGGCGACATTCATGCGGTAAAAGAAATCATGGCA
AAATCGCGCGATATTGGTATGCAAACGTTCGACCAATCGCTGTTTGATTTGTATGAAGCCGATTTGATTTCGTATGACGA
TGCCTTGCGTAATGCTGACTCGGTAAACGATTTGCGCCTGCAAATTCAGTTAAACAGCAAGAAAAACGCAACAGCTGGTT
CGGGCGGCGTATTGGACGGCTTGTCTTTGACCGATTACGCACCGCCAGAAGCAGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.523

89.34

0.523
  pilU Acinetobacter baylyi ADP1

58.239

89.34

0.52
  pilU Vibrio cholerae strain A1552

53.406

93.147

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.162

93.909

0.396
  pilT Legionella pneumophila strain Lp02

43.544

84.518

0.368
  pilT Legionella pneumophila strain ERS1305867

43.544

84.518

0.368

Multiple sequence alignment