Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LHA_RS07590 Genome accession   NZ_LN681225
Coordinates   1689961..1691619 (-) Length   552 a.a.
NCBI ID   WP_045106007.1    Uniprot ID   A0A0A8UV86
Organism   Legionella hackeliae strain ATCC 35250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1684961..1696619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LHA_RS17295 (LHA_1625) - 1685210..1685422 (-) 213 WP_231861995.1 hypothetical protein -
  LHA_RS07575 (LHA_1626) - 1685530..1687899 (-) 2370 WP_045106005.1 ankyrin repeat domain-containing protein -
  LHA_RS07580 (LHA_1627) - 1687998..1688858 (-) 861 WP_045106006.1 A24 family peptidase -
  LHA_RS07585 (LHA_1628) pilC 1688865..1689968 (-) 1104 WP_052673635.1 type II secretion system F family protein Machinery gene
  LHA_RS07590 (LHA_1629) pilB 1689961..1691619 (-) 1659 WP_045106007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LHA_RS07595 (LHA_1630) - 1691724..1691951 (+) 228 WP_045106008.1 hypothetical protein -
  LHA_RS07600 (LHA_1631) - 1692042..1692782 (+) 741 WP_045106009.1 methyltransferase domain-containing protein -
  LHA_RS07605 (LHA_1632) - 1692873..1693199 (+) 327 WP_045107465.1 DUF1820 family protein -
  LHA_RS07610 (LHA_1633) - 1693224..1693784 (+) 561 WP_045106010.1 hypoxanthine-guanine phosphoribosyltransferase -
  LHA_RS07615 (LHA_1634) - 1693817..1694956 (-) 1140 WP_045106011.1 HlyD family type I secretion periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 61914.25 Da        Isoelectric Point: 6.5654

>NTDB_id=1113925 LHA_RS07590 WP_045106007.1 1689961..1691619(-) (pilB) [Legionella hackeliae strain ATCC 35250]
MERIKQESPLPLVAHLLLKSNLMSREKIVQCQHHAMASQQTLQDYLLANEMVSEQQLSLLLSTHFALQYLDLDSIEMDSI
PWRILDAKLMHQLRVVPLMIRENHLILGTDDPSRQSTLKEVQFYTSLSASFILVEASKLTRLIEKINQRKLSEGPRLHSA
SLMSNNVSMESTESDDDSSIVHFVDQIFFDAIKRKVSDIHFELYDDRYRIRYRLDGILIETATPSPDLAKRITARIKIMS
NLDISERRIPQDGRFNVSFNEGSSRDCRVNICPTINGEKVVVRILNPDTSMIRIDELGFNALQKKYFLEAIAKPQGIILV
TGPTGSGKTVSLYTALSMLNTCEKNISTAEDPVEIKVSGINQVNINPKVGLTFANVLRSFLRQDPDIIMIGEIRDVETAE
IAVKAAQTGHLVLSTLHTNSAAETLTRLNSMGISSFDLGSSINLLIAQRLVRRLCSYCKQAHVETERDTASVYKAVGCNQ
CMNGYHGRIGIFEVMPVTSTIQQLIISGSPPVEILKQAQEEGMLTVYQAGLEKIKEGLTTIEEVNRVTVRND

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=1113925 LHA_RS07590 WP_045106007.1 1689961..1691619(-) (pilB) [Legionella hackeliae strain ATCC 35250]
ATGGAAAGAATAAAGCAAGAATCACCATTGCCTCTCGTTGCTCATTTGCTTCTCAAGAGCAATCTAATGAGTCGGGAAAA
AATTGTACAATGTCAACATCATGCGATGGCATCGCAGCAAACACTTCAGGATTATCTTCTAGCAAATGAGATGGTCTCTG
AGCAACAATTGTCTCTATTGTTATCCACGCATTTTGCCCTCCAGTATTTGGATTTGGATTCTATCGAGATGGACTCCATT
CCCTGGCGTATTCTTGATGCAAAGCTAATGCATCAATTGCGTGTAGTACCTCTGATGATACGGGAGAATCATTTGATTCT
AGGCACTGACGATCCTAGTCGCCAATCAACTCTTAAAGAGGTTCAATTCTATACAAGCTTATCAGCCTCCTTTATTCTTG
TTGAAGCGTCAAAATTAACCAGACTGATTGAAAAAATTAATCAGCGAAAACTAAGCGAAGGCCCAAGGCTTCATTCTGCT
TCTCTCATGTCAAATAACGTATCCATGGAAAGCACTGAAAGTGACGATGATTCTTCAATTGTACATTTTGTAGATCAGAT
TTTTTTTGATGCAATTAAAAGGAAAGTGTCGGACATTCATTTTGAACTCTATGACGATAGATATCGCATTCGCTATCGCC
TGGATGGTATTTTAATTGAAACCGCTACCCCCTCACCTGATTTGGCTAAAAGAATAACAGCGCGAATCAAAATCATGTCT
AACCTTGATATTTCTGAGCGCCGTATTCCACAGGATGGACGTTTTAACGTTTCATTTAATGAAGGCTCTTCAAGAGACTG
TCGGGTGAATATCTGCCCTACTATCAACGGGGAAAAAGTGGTGGTTCGTATTCTTAATCCGGATACTTCCATGATTAGGA
TTGATGAGTTGGGTTTTAATGCACTGCAAAAAAAATATTTCCTGGAAGCCATTGCTAAACCTCAGGGAATAATTTTAGTG
ACTGGTCCTACCGGCAGTGGCAAAACAGTTTCTTTATACACAGCACTTAGTATGTTAAACACCTGTGAGAAAAATATTTC
TACAGCGGAGGATCCCGTTGAAATAAAAGTATCCGGTATTAATCAAGTTAATATTAACCCGAAAGTAGGATTAACGTTTG
CTAATGTCCTGCGCTCTTTTCTACGACAAGACCCAGATATCATTATGATAGGAGAAATTCGTGATGTTGAAACCGCAGAA
ATTGCCGTTAAAGCAGCACAAACAGGACATTTGGTCTTGTCTACTTTGCACACTAATAGTGCTGCAGAAACGTTGACTCG
ATTAAACAGCATGGGTATTTCATCATTTGATCTGGGAAGCTCTATAAATCTTTTAATCGCTCAGCGTCTGGTCAGAAGAT
TATGTTCTTATTGCAAGCAAGCACATGTGGAAACTGAAAGGGACACGGCTAGTGTCTATAAGGCAGTTGGATGCAATCAA
TGCATGAATGGCTATCACGGTCGTATTGGAATATTTGAAGTCATGCCTGTTACATCAACGATTCAACAACTAATTATTTC
CGGTAGCCCTCCTGTTGAAATTCTTAAGCAAGCACAAGAAGAAGGAATGCTAACTGTGTATCAAGCCGGCCTTGAAAAAA
TTAAAGAAGGTCTAACCACCATCGAAGAAGTGAATCGAGTGACAGTACGTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8UV86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.697

100

0.553
  pilB Acinetobacter baumannii D1279779

45.504

100

0.458
  pilB Acinetobacter baylyi ADP1

44.326

100

0.453
  pilB Vibrio cholerae strain A1552

44.925

96.377

0.433
  pilB Vibrio parahaemolyticus RIMD 2210633

45.094

96.014

0.433
  pilB Vibrio campbellii strain DS40M4

44.151

96.014

0.424
  pilF Neisseria gonorrhoeae MS11

43.389

97.283

0.422
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

95.471

0.382
  pilF Thermus thermophilus HB27

36.347

100

0.364

Multiple sequence alignment