Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACNRMP_RS07490 Genome accession   NZ_JBLWDC010000001
Coordinates   1634827..1636554 (+) Length   575 a.a.
NCBI ID   WP_028853661.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PD 7123     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629827..1641554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNRMP_RS07460 (ACNRMP_07460) obgE 1629880..1630980 (-) 1101 WP_028853659.1 GTPase ObgE -
  ACNRMP_RS07465 (ACNRMP_07465) rpmA 1631158..1631418 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACNRMP_RS07470 (ACNRMP_07470) rplU 1631456..1631767 (-) 312 WP_011002728.1 50S ribosomal protein L21 -
  ACNRMP_RS07475 (ACNRMP_07475) ispB 1632046..1633014 (+) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  ACNRMP_RS07485 (ACNRMP_07485) - 1633302..1634579 (+) 1278 WP_028853660.1 HlyC/CorC family transporter -
  ACNRMP_RS07490 (ACNRMP_07490) pilF 1634827..1636554 (+) 1728 WP_028853661.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNRMP_RS07495 (ACNRMP_07495) pilC 1636599..1637864 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  ACNRMP_RS07500 (ACNRMP_07500) - 1637867..1638748 (+) 882 WP_028853662.1 prepilin peptidase -
  ACNRMP_RS07505 (ACNRMP_07505) coaE 1638761..1639375 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  ACNRMP_RS07510 (ACNRMP_07510) zapD 1639493..1640251 (+) 759 WP_016723794.1 cell division protein ZapD -
  ACNRMP_RS07515 (ACNRMP_07515) yacG 1640262..1640477 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  ACNRMP_RS07520 (ACNRMP_07520) - 1640470..1640931 (-) 462 WP_028853663.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63710.55 Da        Isoelectric Point: 7.1546

>NTDB_id=1113377 ACNRMP_RS07490 WP_028853661.1 1634827..1636554(+) (pilF) [Ralstonia pseudosolanacearum strain PD 7123]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQEATQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1113377 ACNRMP_RS07490 WP_028853661.1 1634827..1636554(+) (pilF) [Ralstonia pseudosolanacearum strain PD 7123]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCACCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCACTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGATCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGTCGAGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.148

93.913

0.499
  pilB Acinetobacter baumannii D1279779

49.738

99.652

0.496
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.964

96.696

0.435
  pilB Vibrio campbellii strain DS40M4

44.649

94.261

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

35.574

100

0.377
  pilF Thermus thermophilus HB27

38.256

97.739

0.374

Multiple sequence alignment