Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHN93_RS15965 Genome accession   NZ_HG999362
Coordinates   3684300..3686036 (-) Length   578 a.a.
NCBI ID   WP_104600103.1    Uniprot ID   -
Organism   Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 1494 isolated from C. illinoinensis     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3679300..3691036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN93_RS15955 (XCY_003190) - 3680642..3682255 (+) 1614 WP_211920720.1 HAMP domain-containing sensor histidine kinase -
  KHN93_RS15960 (XCY_003191) pilR 3682448..3683842 (+) 1395 WP_211920722.1 sigma-54 dependent transcriptional regulator Regulator
  KHN93_RS15965 (XCY_003192) pilB 3684300..3686036 (-) 1737 WP_104600103.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHN93_RS21725 (XCY_003194) - 3686666..3687118 (-) 453 WP_104600101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KHN93_RS15980 (XCY_003195) pilC 3687472..3688725 (+) 1254 WP_104600100.1 type II secretion system F family protein Machinery gene
  KHN93_RS15985 (XCY_003196) - 3688733..3689596 (+) 864 WP_016849610.1 A24 family peptidase -
  KHN93_RS15990 (XCY_003197) coaE 3689610..3690233 (+) 624 WP_211920724.1 dephospho-CoA kinase -
  KHN93_RS15995 (XCY_003198) - 3690333..3690488 (+) 156 Protein_3141 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62633.80 Da        Isoelectric Point: 5.5966

>NTDB_id=1112638 KHN93_RS15965 WP_104600103.1 3684300..3686036(-) (pilB) [Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 1494 isolated from C. illinoinensis]
MSIAISTNLVGITGLARRLVQDGALDEVAARAAMDQAAQAKVPLPQWFADRKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1112638 KHN93_RS15965 WP_104600103.1 3684300..3686036(-) (pilB) [Xanthomonas arboricola pv. juglandis isolate Xanthomonas arboricola pv. juglandis CPBF 1494 isolated from C. illinoinensis]
ATGAGCATCGCAATCAGCACCAACTTGGTCGGTATCACTGGGCTGGCGCGTCGTCTTGTGCAAGATGGTGCTCTTGATGA
AGTGGCGGCCCGTGCTGCAATGGATCAGGCTGCGCAAGCGAAGGTGCCCCTCCCGCAATGGTTCGCGGACAGGAAGCTGG
TGTCCGCGGCACAACTTGCGGCGGCTAATGCAGTCGAGTTCGGCATGCCTCTAATGGACGTTTCCGTCTTCGACGCCAGC
CAAAACGCCATTAAGCTAGTCAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACTAGGGCGCTGGACGACATCAAGTTTCATACCAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGATCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCTTCGCTCGGT
GACGACGACGAGGGGATGGGCGATCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGTTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCCTACGAAGACGACTACCGGGTGCGGCTGCGGATCGACGGGCTGCTGAAGAACGTGGCCAAGGCACCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGACATTGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACTTTGCCGACCCTGTTCGGCGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCTGACCAGCAGAAG
CTGTTCCTGGATGCGATTCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTACACGGCACTGGGCATCCTCAACGACGAGACGCGCAATATCTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCCG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTACGCTCGTTCTTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCCATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGTACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTC
AAGGCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAACCGGGTGACGAAAGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.27

0.547
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilB Vibrio cholerae strain A1552

48.789

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.583

97.924

0.446

Multiple sequence alignment