Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACMCNI_RS08450 Genome accession   NZ_AP038791
Coordinates   1821182..1821619 (-) Length   145 a.a.
NCBI ID   WP_226691633.1    Uniprot ID   -
Organism   Rodentibacter sp. THUN1657     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1816182..1826619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCNI_RS08420 (THUN1657_16170) - 1816789..1817070 (-) 282 WP_412763599.1 GNAT family N-acetyltransferase -
  ACMCNI_RS08425 (THUN1657_16180) yacG 1817067..1817273 (-) 207 WP_226691637.1 DNA gyrase inhibitor YacG -
  ACMCNI_RS08430 (THUN1657_16190) coaE 1817266..1817886 (-) 621 WP_226692923.1 dephospho-CoA kinase -
  ACMCNI_RS08435 (THUN1657_16200) pilD 1817886..1818587 (-) 702 WP_226691636.1 prepilin peptidase Machinery gene
  ACMCNI_RS08440 (THUN1657_16210) pilC 1818584..1819804 (-) 1221 WP_226691635.1 type II secretion system F family protein Machinery gene
  ACMCNI_RS08445 (THUN1657_16220) pilB 1819806..1821185 (-) 1380 WP_226691634.1 GspE/PulE family protein Machinery gene
  ACMCNI_RS08450 (THUN1657_16230) pilA 1821182..1821619 (-) 438 WP_226691633.1 prepilin peptidase-dependent pilin Machinery gene
  ACMCNI_RS08455 (THUN1657_16240) ampD 1821735..1822298 (+) 564 WP_226691632.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMCNI_RS08460 (THUN1657_16250) rppH 1822815..1823399 (+) 585 WP_226691631.1 RNA pyrophosphohydrolase -
  ACMCNI_RS08465 (THUN1657_16260) lgt 1823531..1824331 (+) 801 WP_374707287.1 prolipoprotein diacylglyceryl transferase -
  ACMCNI_RS08470 (THUN1657_16270) - 1824455..1825306 (+) 852 WP_226691630.1 thymidylate synthase -
  ACMCNI_RS08475 (THUN1657_16280) tadA 1825318..1825824 (+) 507 WP_255618266.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15020.27 Da        Isoelectric Point: 8.4769

>NTDB_id=111257 ACMCNI_RS08450 WP_226691633.1 1821182..1821619(-) (pilA) [Rodentibacter sp. THUN1657]
MKLTSQRTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAMSELLQAATPFKSDVELCVYSTGATANCSGGSNGIA
ADITSPKGYVKGISTNAGVITVVGNGALDGVQYTLTATGDHSRGISWSTTCNGDTSLFPAGFCTK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=111257 ACMCNI_RS08450 WP_226691633.1 1821182..1821619(-) (pilA) [Rodentibacter sp. THUN1657]
ATGAAACTCACCTCTCAACGCACATTGAAAAAAGGATTTACACTAATCGAATTAATGATCGTGATAGCCATCATCGCGAT
TCTCGCGACAATTGCAATTCCCTCTTATCAGAATTATACAAAAAAGGCGGCGATGTCTGAATTGTTACAAGCCGCAACCC
CTTTTAAATCCGACGTGGAACTCTGCGTTTATAGCACAGGAGCAACCGCAAATTGCAGCGGCGGTTCAAACGGTATCGCT
GCCGATATTACCTCACCAAAAGGCTATGTGAAAGGTATTAGTACTAACGCCGGCGTTATTACTGTTGTCGGCAACGGCGC
TTTAGACGGTGTCCAATATACATTAACCGCCACCGGTGATCATTCTAGAGGAATAAGCTGGTCTACAACCTGTAACGGTG
ATACCTCCTTATTTCCGGCAGGATTCTGTACAAAATGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

72.222

99.31

0.717
  pilA Haemophilus influenzae Rd KW20

69.444

99.31

0.69
  pilA Glaesserella parasuis strain SC1401

57.746

97.931

0.566
  pilA Vibrio campbellii strain DS40M4

38.71

100

0.414

Multiple sequence alignment